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IGFBP6 Antibody (YA3076)(PBS only)

(Synonyms: IBP6)

IGFBP6 Antibody (YA3076) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
  • IGFBP6 Antibody (YA3076)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

IGFBP6 Antibody (YA3076) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP6.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Immunogen

Recombinant protein of human IGFBP6

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
    Western blot analysis of extracts from U937(lane 2(20ug) , Ramos(lane 3(20ug) and SH-SY5Y(lane 4(20ug) using IGFBP6 Antibody (HY-P83331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
    Western blot analysis was performed on extracts from HaCaT (lane 1, 15 μg), U-87MG (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), and NCI-H1299 (lane 5, 15 μg) using IGFBP6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    IGFBP6 is an IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration[1].

  • Subcellular Localization

    Secreted

  • Subunit

    Interacts (via C-terminal domain) with PHB2

  • SwissProt ID

    P24592

  • Gene ID
    3489 [NCBI]
  • Synonyms

    IBP6

  • Research Field

    Neuroscience

IGFBP6 Antibody (YA3076)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U937(lane 2(20ug) , Ramos(lane 3(20ug) and SH-SY5Y(lane 4(20ug) using IGFBP6 Antibody (HY-P83331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HaCaT (lane 1, 15 μg), U-87MG (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), and NCI-H1299 (lane 5, 15 μg) using IGFBP6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IGFBP6 Antibody (YA3076)(PBS only) Product Image
    1/16
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
  • IGFBP6 Antibody (YA3076)(PBS only) Product Image
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U937(lane 2(20ug) , Ramos(lane 3(20ug) and SH-SY5Y(lane 4(20ug) using IGFBP6 Antibody (HY-P83331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HaCaT (lane 1, 15 μg), U-87MG (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), and NCI-H1299 (lane 5, 15 μg) using IGFBP6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
  • Experimental Validation Results for IGFBP6 Antibody (YA3076)(PBS only)
  • IGFBP6 Antibody (YA3076)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U937(lane 2(20ug) , Ramos(lane 3(20ug) and SH-SY5Y(lane 4(20ug) using IGFBP6 Antibody (HY-P83331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IGFBP6 Antibody (YA3076)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HaCaT (lane 1, 15 μg), U-87MG (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), and NCI-H1299 (lane 5, 15 μg) using IGFBP6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IGFBP6 Antibody (YA3076)(PBS only) Product Image WB
  • IGFBP6 Antibody (YA3076)(PBS only) Product Image WB