2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. Cdc25C Antibody (YA4788)

Cdc25C Antibody (YA4788)

製品番号: HY-P85096
User Guide for Antibodies Technical Support

Cdc25C Antibody (YA4788) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cdc25C.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Cdc25C Antibody (YA4788) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cdc25C.
Host

Mouse
Clonality

Monoclonal
分子量

Predicted band size: 53 kDa;

Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human CDC25C expressed in E. Coli.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-1:1000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-200
純度 affinity purified. Conjugation Non-conjugated
Modification Unmodified  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945)
Subcellular Localization：Nucleus
Isoforms & Post-Translational Modification：P30307 has 5 isomers: P30307-1: 53365 Da (predicted); P30307-2: 50122 Da (predicted); P30307-5: Da (predicted); P30307-3: 48851 Da (predicted); P30307-4: 45608 Da (predicted).
Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity
Subunit：Interacts with MAPK14 and 14-3-3 proteins (PubMed:11333986). When phosphorylated on Ser-129 and/or Thr-130, interacts with PLK1 (PubMed:12595692). Interacts with MARK3/C-TAK1 (PubMed:12941695)
Synonyms
CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C
ドキュメンテーション
User Guide for Antibodies (1077 KB)

Cdc25C Antibody (YA4788) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

最近チェックした製品:

オンラインお問い合わせ

Your information is safe with us. * Required Fields.

製品名

  

カスタマ需要量 *

お名前 *

 

タイトル

メールアドレス *

 

電話番号 *

デパートメント

 

組纖名 *

市区町村

都道府県

国或いは地域 *

      

必ず会社名を記載ください。個人への返信は行いません。

備考

バルクお問い合わせ

Inquiry Information

製品名:
Cdc25C Antibody (YA4788)
製品番号:
HY-P85096
数量:
MCE 日本正規代理店:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

製品名

  

Lot #

お名前 *

 

国或いは地域 *

メールアドレス *

     

電話番号 *

 

組纖名 *

備考

Request for HNMR Report

We have received your request and will respond to you as soon as possible.