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p40 Antibody (YA1500)

(Synonyms: p40; RAB9p40; RABEPK; RGD1310612; RP11 65N13.1; RP23-446N16.2)
Customer Review

Based on 1 Customer Validation

p40 Antibody (YA1500) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p40.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for p40 Antibody (YA1500)
  • p40 Antibody (YA1500) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

p40 Antibody (YA1500) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p40.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 41 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Species Reactivity Database

Entrez Gene: 10244 Human

SwissProt: Q7Z6M1 Human

Immunogen

Recombinant protein of human p40

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for p40 Antibody (YA1500)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), HepG2 (lane 4), U87 (lane 5), Jurkat (lane 6), and A549 (lane 7) using p40 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    p40 is a Rab9 effector required for endosome to trans-Golgi network (TGN) transport

  • Subcellular Localization

    Cytoplasm; Endosome membrane

  • Isoforms & Post-Translational Modification

    Q7Z6M1 has 2 isomers: Q7Z6M1-1: 40565 Da (predicted); Q7Z6M1-2: 34841 Da (predicted).
    Phosphorylated on Ser residues by PIKFYVE

  • Subunit

    Interacts with PIKFYVE; the interaction recruits RABEPK to the endosomal membrane (PubMed:14530284). Interacts with RAB9 in its GTP-bound conformation (PubMed:16769818, PubMed:9230071)

  • SwissProt ID

    Q7Z6M1

  • Gene ID
    10244 [NCBI]
  • Synonyms

    p40; RAB9p40; RABEPK; RGD1310612; RP11 65N13.1; RP23-446N16.2

  • Research Field

    Signal Transduction

p40 Antibody (YA1500) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), HepG2 (lane 4), U87 (lane 5), Jurkat (lane 6), and A549 (lane 7) using p40 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • p40 Antibody (YA1500) Product Image
    1/16
  • Experimental Validation Results for p40 Antibody (YA1500)
  • p40 Antibody (YA1500) Product Image
  • Experimental Validation Results for p40 Antibody (YA1500)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), HepG2 (lane 4), U87 (lane 5), Jurkat (lane 6), and A549 (lane 7) using p40 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for p40 Antibody (YA1500)
  • p40 Antibody (YA1500) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), HepG2 (lane 4), U87 (lane 5), Jurkat (lane 6), and A549 (lane 7) using p40 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • p40 Antibody (YA1500) Product Image WB