TET2 Antibody (YA4336)(PBS only)

Art. -Nr.: HY-P84639A: Data Sheet User Guide for Antibodies
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TET2 Antibody (YA4336) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TET2.

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Größe		Verfügbarkeit
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100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

TET2 Antibody (YA4336) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TET2.

Host

Mouse

Molekulargewicht

Predicted band size: 224 kDa;

Observed band size: 130/250 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6N021

Gene ID

54790 [NCBI]

Immunogen

Purified recombinant fragment of human TET2 aa 1883-2002.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Reinheit	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of affected by myelodysplastic syndromes

Isoforms & Post-Translational Modification：Q6N021 has 3 isomers: Q6N021-1: 223811 Da (predicted); Q6N021-2: 130254 Da (predicted); Q6N021-3: 133481 Da (predicted).
May be glycosylated. It is unclear whether interaction with OGT leads to GlcNAcylation. According to a report, it is not GlcNAcylated by OGT (PubMed:23353889). In contrast, another group reports GlcNAcylation by OGT in mouse ortholog;Monoubiquitinated at Lys-1299 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex; this modification promotes binding to DNA;Acetylated (PubMed:39567688). Deacetylase HDAC6 acts as a valine sensor by binding to valine through its primate-specific SE14 repeat region and deacetylates TET2 following valine deprivation which promotes TET2-dependent DNA demethylation (PubMed:39567688)

Subunit：Interacts with HCFC1 (PubMed:23353889). Interacts with OGT (PubMed:23222540, PubMed:23353889). Interacts with PROSER1; this interaction mediates TET2 O-GlcNAcylation and stability by promoting the interaction between OGT and TET2 (PubMed:34667079). Directly interacts (via C-terminus) with the DCAF1 component of the CRL4(VprBP) E3 ubiquitin-protein ligase complex (PubMed:24357321, PubMed:25557551)

Synonyms

MDS; KIAA1546

Dokumentation

User Guide for Antibodies (1077 KB)