15-LO2 Antibody (YA8984)
(Synonyms: Polyunsaturated fatty acid lipoxygenase ALOX15B, 15-lipoxygenase 2, Arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 15-LOX-2, 15-LOX-B, ALOX15B)
Based on 1 Customer Validation
15-LO2 Antibody (YA8984) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to 15-LO2.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
15-LO2 Antibody (YA8984) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to 15-LO2.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 76 kDa
OMIM: 603697
A synthesized peptide derived from human 15-LO2.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
15-LO2 is a Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist. Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates. Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively down-regulate the immune response and have anti-aggregation properties with platelets. In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids. Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea.
Induction: Up-regulated by UV-irradiation. -
Isoforms & Post-Translational Modification
15-LO2 has 4 isoforms, O15296-1: amino acid length is 676, molecular weight is 75857 Da (predicted); O15296-2: amino acid length is 602, molecular weight is 67345 Da (predicted); O15296-3: amino acid length is 617, molecular weight is 69136 Da (predicted); O15296-4: amino acid length is 647, molecular weight is 72523 Da (predicted).
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SwissProt ID
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Synonyms
Polyunsaturated fatty acid lipoxygenase ALOX15B, 15-lipoxygenase 2, Arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 15-LOX-2, 15-LOX-B, ALOX15B
Documentation
References
[1]. Kilty I, et al. Differential characteristics of human 15-lipoxygenase isozymes and a novel splice variant of 15S-lipoxygenase. Eur J Biochem. 1999 Nov;266(1):83-93. [Content Brief]
[2]. Jisaka M, et al. Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2. J Biol Chem. 2000 Jan 14;275(2):1287-93. [Content Brief]
[3]. Bhatia B, et al. Subcellular localization and tumor-suppressive functions of 15-lipoxygenase 2 (15-LOX2) and its splice variants. J Biol Chem. 2003 Jul 4;278(27):25091-100. [Content Brief]
[4]. Prusakiewicz JJ, et al. Oxidative metabolism of lipoamino acids and vanilloids by lipoxygenases and cyclooxygenases. Arch Biochem Biophys. 2007 Aug 15;464(2):260-8. [Content Brief]
[5]. Turman MV, et al. Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurine. Biochemistry. 2008 Mar 25;47(12):3917-25. [Content Brief]
[6]. Horn T, et al. Functional characterization of genetic enzyme variations in human lipoxygenases. Redox Biol. 2013;1(1):566-77. [Content Brief]
[7]. Kobe MJ, et al. The structure of human 15-lipoxygenase-2 with a substrate mimic. J Biol Chem. 2014 Mar 21;289(12):8562-9. [Content Brief]
[8]. Perry SC, et al. 15-Lipoxygenase-1 biosynthesis of 7S,14S-diHDHA implicates 15-lipoxygenase-2 in biosynthesis of resolvin D5. J Lipid Res. 2020 Jul;61(7):1087-1103. [Content Brief]
[9]. Brash AR, et al. Discovery of a second 15S-lipoxygenase in humans. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6148-52. [Content Brief]
[10]. Kozak KR, et al. 15-Lipoxygenase metabolism of 2-arachidonylglycerol. Generation of a peroxisome proliferator-activated receptor alpha agonist. J Biol Chem. 2002 Jun 28;277(26):23278-86. [Content Brief]
[11]. Bender G, et al. Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart: IMPLICATIONS FOR MURINE MODELS OF ATHEROSCLEROSIS. J Biol Chem. 2016 Sep 9;291(37):19413-24. [Content Brief]
[12]. Green AR, et al. Strict Regiospecificity of Human Epithelial 15-Lipoxygenase-2 Delineates Its Transcellular Synthesis Potential. Biochemistry. 2016 May 24;55(20):2832-40. [Content Brief]
[13]. Tang S, et al. Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells. J Biol Chem. 2002 May 3;277(18):16189-201. [Content Brief]
[14]. Danielsson KN, et al. 15-Lipoxygenase-2 expression in human macrophages induces chemokine secretion and T cell migration. Atherosclerosis. 2008 Jul;199(1):34-40. [Content Brief]
[15]. Magnusson LU, et al. Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis. PLoS One. 2012;7(8):e43142. [Content Brief]
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