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Nestin Antibody (YA4643)

(Synonyms: ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.)
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Based on 1 Customer Validation

Nestin Antibody (YA4643) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nestin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP, IF-Tissue, mIHC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Nestin Antibody (YA4643)
  • Nestin Antibody (YA4643) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
mIHC Info
mIHC: Multiplex Immunohistochemical
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:1000-1:5000 1:1000 1:500-1:2000 1:500 1:1000 Use at an assay dependent concentration.

Product Details

Description

Nestin Antibody (YA4643) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nestin.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 268 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 177 kDa
Species Reactivity Database
Immunogen

Recombinant protein within mouse Nestin aa 700-1,000.

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719012

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Nestin Antibody (YA4643)
    Western blot analysis of extracts from Neuro-2a (lane 2(20μg) and Neuro-2a (lane 3(40μg) using Nestin (HY-P84951) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Nestin is required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells

  • Expression


    Tissue_specificity:Central nervous system stem cells
    Positive sample: RD cell lysate, Neuro-2a cell lysate, Mouse brain (P0) tissue lysate, Mouse brain (P7) tissue lysate, Mouse brain tissue lysate, U-87 MG, C2C12, C6, human kidney tissue, mou

  • Isoforms & Post-Translational Modification

    Q6P5H2 has 2 isomers: Q6P5H2-1: 207124 Da (predicted); Q6P5H2-2: 201770 Da (predicted).
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity)

  • Subunit

    Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 (By similarity)

  • SwissProt ID

    Q6P5H2

  • Gene ID
    18008 [NCBI]
  • Synonyms

    ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.

Nestin Antibody (YA4643) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Neuro-2a (lane 2(20μg) and Neuro-2a (lane 3(40μg) using Nestin (HY-P84951) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Nestin Antibody (YA4643) Product Image
    1/16
  • Experimental Validation Results for Nestin Antibody (YA4643)
  • Nestin Antibody (YA4643) Product Image
  • Experimental Validation Results for Nestin Antibody (YA4643)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Neuro-2a (lane 2(20μg) and Neuro-2a (lane 3(40μg) using Nestin (HY-P84951) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nestin Antibody (YA4643)
  • Nestin Antibody (YA4643) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Neuro-2a (lane 2(20μg) and Neuro-2a (lane 3(40μg) using Nestin (HY-P84951) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Nestin Antibody (YA4643) Product Image WB