MAD1L1 Antibody (YA2449)

製品番号: HY-P82704: データシート User Guide for Antibodies
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MAD1L1 Antibody (YA2449) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD1L1.

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容量	価格（税別）	在庫状況	数量
20 μL	$146	Get quote 3 - 4 Weeks 2 - 3 Weeks 4 - 5 Weeks 3 - 4 weeks	Estimated Time of Arrival: December 31
50 μL	$222	Get quote 3 - 4 Weeks 2 - 3 Weeks 4 - 5 Weeks 3 - 4 weeks	Estimated Time of Arrival: December 31
100 μL	$360	Get quote 3 - 4 Weeks 2 - 3 Weeks 4 - 5 Weeks 3 - 4 weeks	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

Synthetic products have potential research and development risk.

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

MAD1L1 Antibody (YA2449) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD1L1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 83 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y6D9

Gene ID

8379 [NCBI]

Immunogen

A synthesized peptide derived from human MAD1 aa50-170/718.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:10049595, PubMed:20133940, PubMed:29162720). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed:29162720); Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas

Subcellular Localization：Nucleus; Chromosome, centromere, kinetochore; Nucleus envelope; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm

Expression：
Tissue_specificity:Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level) ; Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level)

Induction:Increased by p53/TP53

Isoforms & Post-Translational Modification：Q9Y6D9 has 3 isomers: Q9Y6D9-1: 83067 Da (predicted); Q9Y6D9-3: 72286 Da (predicted); Q9Y6D9-4: 77460 Da (predicted).
Phosphorylated; by BUB1 (PubMed:10198256). Become hyperphosphorylated in late S through M phases or after mitotic spindle damage (PubMed:9546394). Phosphorylated; by TTK (PubMed:29162720)

Subunit：Homodimer (PubMed:29162720, PubMed:9546394). Dimerizes via its N- and C- terminal regions (PubMed:29162720). Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex (PubMed:12006501, PubMed:18981471, PubMed:22351768, PubMed:9546394). Interacts with the closed conformation form of MAD2L1 (C-MAD2) and open conformation form of MAD2L1 (O-MAD2) (PubMed:29162720). It is unclear whether MAD1L1 dimerization promotes the conversion of closed to open conformation of MAD2L1 (PubMed:29162720). Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer (PubMed:12006501). Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1 (PubMed:12006501). CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20 (PubMed:12006501). Also able to interact with the BUB1/BUB3 complex (PubMed:10198256). Interacts with NEK2 (PubMed:14978040). Interacts with TTK (PubMed:29162720). Interacts with TPR; the interactions occurs in a microtubule-independent manner (PubMed:18981471, PubMed:19273613, PubMed:20133940). Interacts with IK (PubMed:22351768). Interacts with the viral Tax protein (PubMed:9546394). Interacts with PRAP1 (PubMed:24374861)

Database

Entrez Gene: 8379 Human ; 17120 Mouse ; 680006 Rat

SwissProt: Q9Y6D9 Human ; Q9WTX8 Mouse ;

OMIM: 620189 Human

Research Field

Cell Biology

Synonyms

hMAD1; HsMAD1; MAD1; MAD1L1; PIG9; TP53I9; TXBP181

ドキュメンテーション

User Guide for Antibodies (1077 KB)