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  5. MAD1L1 Antibody (YA2449)

MAD1L1 Antibody (YA2449)

Cat. No.: HY-P82704
User Guide for Antibodies Technical Support

MAD1L1 Antibody (YA2449) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD1L1.

For research use only. We do not sell to patients.

Size Price Stock Quantity
20 μL Get quote 3 - 4 Weeks 2 - 3 Weeks 4 - 5 Weeks 3 - 4 weeks
50 μL Get quote 3 - 4 Weeks 2 - 3 Weeks 4 - 5 Weeks 3 - 4 weeks
100 μL Get quote 3 - 4 Weeks 2 - 3 Weeks 4 - 5 Weeks 3 - 4 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

MAD1L1 Antibody (YA2449) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAD1L1.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 83 kDa;
Observed band size: 80 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human MAD1 aa50-170/718.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:10049595, PubMed:20133940, PubMed:29162720). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed:29162720); Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas
Subcellular Localization：Nucleus; Chromosome, centromere, kinetochore; Nucleus envelope; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm
Expression：
Tissue_specificity:Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level) ; Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level)

Induction:Increased by p53/TP53
Isoforms & Post-Translational Modification：Q9Y6D9 has 3 isomers: Q9Y6D9-1: 83067 Da (predicted); Q9Y6D9-3: 72286 Da (predicted); Q9Y6D9-4: 77460 Da (predicted).
Phosphorylated; by BUB1 (PubMed:10198256). Become hyperphosphorylated in late S through M phases or after mitotic spindle damage (PubMed:9546394). Phosphorylated; by TTK (PubMed:29162720)
Subunit：Homodimer (PubMed:29162720, PubMed:9546394). Dimerizes via its N- and C- terminal regions (PubMed:29162720). Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex (PubMed:12006501, PubMed:18981471, PubMed:22351768, PubMed:9546394). Interacts with the closed conformation form of MAD2L1 (C-MAD2) and open conformation form of MAD2L1 (O-MAD2) (PubMed:29162720). It is unclear whether MAD1L1 dimerization promotes the conversion of closed to open conformation of MAD2L1 (PubMed:29162720). Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer (PubMed:12006501). Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1 (PubMed:12006501). CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20 (PubMed:12006501). Also able to interact with the BUB1/BUB3 complex (PubMed:10198256). Interacts with NEK2 (PubMed:14978040). Interacts with TTK (PubMed:29162720). Interacts with TPR; the interactions occurs in a microtubule-independent manner (PubMed:18981471, PubMed:19273613, PubMed:20133940). Interacts with IK (PubMed:22351768). Interacts with the viral Tax protein (PubMed:9546394). Interacts with PRAP1 (PubMed:24374861)
Database

Entrez Gene: 8379 Human ; 17120 Mouse ; 680006 Rat

SwissProt: Q9Y6D9 Human ; Q9WTX8 Mouse ;

OMIM: 620189 Human

Research Field

Cell Biology
Synonyms
hMAD1; HsMAD1; MAD1; MAD1L1; PIG9; TP53I9; TXBP181
Documentation
User Guide for Antibodies (1077 KB)

MAD1L1 Antibody (YA2449) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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