UHRF1 Antibody (YA4440)
(Synonyms: Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95)UHRF1 Antibody (YA4440) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to UHRF1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
UHRF1 Antibody (YA4440) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to UHRF1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 97 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 90 kDa
Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) functions as a multidomain epigenetic regulator coordinating DNA methylation, histone modifications, and protein ubiquitination[1][2]. Mechanistically, UHRF1 interacts with DNMT1 to maintain DNA methylation during replication and recruits histone-modifying enzymes, ensuring stable epigenetic silencing of target genes[3][4][5]. Through these interactions, UHRF1 regulates cell cycle progression, redox homeostasis, and chromatin accessibility in diverse cell types, including vascular smooth muscle cells (VSMCs) and retinal cells[6][7][8]. Compared with UHRF2, UHRF1 uniquely maintains daughter strand DNA methylation and interacts with a broader set of epigenetic effectors, producing distinct functional outcomes[1][9]. In disease contexts, UHRF1 overexpression drives tumorigenesis in multiple cancers, promotes epithelial-mesenchymal transition in renal fibrosis, and mediates chemotherapy resistance via modulation of apoptotic and oxidative stress pathways[2][6][10][11][12][13][8]. In vascular pathology, UHRF1 controls VSMC plasticity by repressing cell cycle inhibitors and prodifferentiation genes, linking epigenetic regulation to cardiovascular remodeling[8]. Functionally, UHRF1 also suppresses retrotransposon activation and regulates innate immune responses, highlighting its epigenetic and non-DNA methylation-dependent roles[12]. For experimental applications, chemical inhibitors and small molecules targeting UHRF1’s SRA or RING domains have been used to modulate DNA methylation, gene expression, and apoptosis in cellular and animal models[14][15][16].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:It is expressed in the thymus, bone marrow, testes, lungs, and heart. It is overexpressed in breast cancer.
Induction:Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a p53/TP53 and CDKN1A-dependent way. Induced by E2F1 transcription factor -
Isoforms & Post-Translational Modification
Q96T88 has 2 isomers: Q96T88-1: 89814 Da (predicted); Q96T88-2: 91116 Da (predicted).
Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome;Ubiquitinated; which leads to proteasomal degradation. Autoubiquitinated; interaction with USP7 leads to deubiquitination and prevents degradation. Ubiquitination and degradation takes place during M phase, when phosphorylation at Ser-639 prevents interaction with USP7 and subsequent deubiquitination. Polyubiquitination may be stimulated by DNA damage -
Subunit
Interacts with DNMT3A and DNMT3B (By similarity). Interacts with DNMT1; the interaction is direct. Interacts with USP7; leading to its deubiquitination. Interacts with histone H3. Interacts with HDAC1, but not with HDAC2. Interacts with BLTP3A. Interacts with PML. Interacts with EHMT2. Binds hemimethylated CpG containing oligonucleotides. Interacts with ZNF263; recruited to the SIX3 promoter along with other proteins involved in chromatin modification and transcriptional corepression where it contributes to transcriptional repression (PubMed:32051553). Interacts with UHRF2 (PubMed:30335751). Interacts with FANCD2 (PubMed:30335751). Interacts with TET1 isoform 2; this interaction induces the recruitment of TET1 isoform 2 to replicating heterochromatin (By similarity)
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SwissProt ID
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Synonyms
Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Documentation
[1]. Unoki M, et al. The UHRF protein family in epigenetics, development, and carcinogenesis. Proc Jpn Acad Ser B Phys Biol Sci. 2022;98(8):401-415. [Content Brief]
[2]. Lin S, et al. UHRF1/DNMT1-MZF1 axis-modulated intragenic site-specific CpGI methylation confers divergent expression and opposing functions of PRSS3 isoforms in lung cancer. Acta Pharm Sin B. 2023 May;13(5):2086-2106. [Content Brief]
[3]. Kim JK, et al. UHRF1 downmodulation enhances antitumor effects of histone deacetylase inhibitors in retinoblastoma by augmenting oxidative stress-mediated apoptosis. Mol Oncol. 2020 Feb;14(2):329-346. [Content Brief]
[4]. Chen X, et al. Overexpression of UHRF1 promoted the proliferation of vascular smooth cells via the regulation of Geminin protein levels. Biosci Rep. 2019 Feb 26;39(2):BSR20181341. [Content Brief]
[5]. Zhang Y, et al. UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer. Cell Death Dis. 2026 Feb 27;17(1):286. [Content Brief]
[6]. Wang X, et al. UHRF2 accumulates in early G1-phase after serum stimulation or mitotic exit to extend G1 and total cell cycle length. Cell Cycle. 2024 Mar;23(5):613-627. [Content Brief]
[7]. Li HG, et al. Uhrf1 regulates H3K9me2 modification of mTOR to inhibit the effect of autophagy in myocardial ischemia-reperfusion injury. Aging (Albany NY). 2021 Mar 19;13(7):9704-9718. [Content Brief]
[8]. Yang L, et al. UHRF1 promotes epithelial-mesenchymal transition mediating renal fibrosis by activating the TGF-β/SMAD signaling pathway. Sci Rep. 2025 Jan 27;15(1):3346. [Content Brief]
[9]. Kim A, et al. Oncogenic Roles of UHRF1 in Cancer. Epigenomes. 2024 Jul 1;8(3):26. [Content Brief]
[10]. Choudhry H, et al. Targeting microRNA/UHRF1 pathways as a novel strategy for cancer therapy. Oncol Lett. 2018 Jan;15(1):3-10. [Content Brief]
[11]. Elia L, et al. UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease. J Clin Invest. 2018 Jun 1;128(6):2473-2486. [Content Brief]
[12]. Alhosin M, et al. Signalling pathways in UHRF1-dependent regulation of tumor suppressor genes in cancer. J Exp Clin Cancer Res. 2016 Nov 14;35(1):174. [Content Brief]
[13]. Li J, et al. Activated MEK/ERK Pathway Drives Widespread and Coordinated Overexpression of UHRF1 and DNMT1 in Cancer cells. Sci Rep. 2019 Jan 29;9(1):907. [Content Brief]
[14]. Peng Y, et al. PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stability. Cell Death Discov. 2023 Oct 3;9(1):367. [Content Brief]
[15]. Alhosin M, et al. Induction of apoptosis by thymoquinone in lymphoblastic leukemia Jurkat cells is mediated by a p73-dependent pathway which targets the epigenetic integrator UHRF1. Biochem Pharmacol. 2010 May 1;79(9):1251-60. [Content Brief]
[16]. Irwin RE, et al. The UHRF1 protein is a key regulator of retrotransposable elements and innate immune response to viral RNA in human cells. Epigenetics. 2023 Dec;18(1):2216005. [Content Brief]
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