UBE1L Antibody (YA5097)
(Synonyms: UBA7; UBE1L; UBE2; Ubiquitin-like modifier-activating enzyme 7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog)UBE1L Antibody (YA5097) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to UBE1L.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB; IHC-P; IHC-F; FC; IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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FC
FC: Flow Cytometry
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-200 | 1:50-100 | 1:100-200 |
Product Details
UBE1L Antibody (YA5097) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to UBE1L.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 112 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 112 kDa
A synthesized peptide derived from human UBA7. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
UBE1L is an E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation. Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus. For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition. ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines
Induction:By DNA damage (PubMed:27545325) . Upon interferon-alpha exposure (PubMed:28035359) -
Subunit
(Microbial infection) Interacts with human cytomegalovirus proteins NEC2/UL50 and UL26; these interactions inhibit ISGylation and cause proteasomal degradation of UBA7
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SwissProt ID
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Synonyms
UBA7; UBE1L; UBE2; Ubiquitin-like modifier-activating enzyme 7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog
Documentation
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