CCK-4 Antibody (YA4771)

Cat. No.: HY-P85079: Data Sheet User Guide for Antibodies
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CCK-4 Antibody (YA4771) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CCK-4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CCK-4 Antibody (YA4771) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CCK-4.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 118 kDa;

Species Reactivity

Human

SwissProt ID

Q13308

Gene ID

5754 [NCBI]

Immunogen

Purified recombinant fragment of human CCK-4 expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis

Subcellular Localization：Membrane; Single-pass type I membrane protein; Cell junction

Expression：
Tissue_specificity:It is highly expressed in the lungs, liver, pancreas, kidneys, placenta, and melanocytes. It is weakly expressed in the thyroid, ovaries, brain, heart, and skeletal muscle. It is also expressed in erythroleukemia cells, but not in the colon.

Induction:Higher expression in cell lines established from normal non-tumorigenic tissues compared to cell lines established from highly metastatic invasive carcinomas (at protein level)

Isoforms & Post-Translational Modification：Q13308 has 6 isomers: Q13308-1: 118392 Da (predicted); Q13308-2: 113805 Da (predicted); Q13308-3: 103581 Da (predicted); Q13308-4: 112261 Da (predicted); Q13308-5: 89764 Da (predicted); Q13308-6: 119197 Da (predicted).
MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway

Subunit：Interacts with CTNNB1

Synonyms

PTK7; CCK4; Inactive tyrosine-protein kinase 7; Colon carcinoma kinase 4; CCK-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; Tyrosine-protein kinase-like 7

Documentation

User Guide for Antibodies (1077 KB)