MUC13 Antibody (YA4495)

Cat. No.: HY-P84798: Data Sheet COA
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User Guide for Antibodies
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MUC13 Antibody (YA4495) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MUC13.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

MUC13 Antibody (YA4495) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MUC13.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9H3R2

Gene ID

56667 [NCBI]

Immunogen

Purified recombinant fragment of human MUC13 (AA: extra 19-238) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH/3T3(lane 1(40μg) ),SW480(lane 2(40μg) ),HT-29(lane 3(40μg) ),LOVO(lane 4(40μg) ),SW620(lane 5(40μg) )and HCT116(lane 6(40μg) ) using MUC13 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Apical cell membrane; Secreted

Expression：
Tissue_specificity:This gene is highly expressed in a variety of epithelial tissues, especially in the gastrointestinal and respiratory epithelial tissues, such as the large intestine and trachea, followed by the kidneys, small intestine, appendix and stomach.

Subunit：Homodimer of beta subunits

Synonyms

DRCC1; MUC-13

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hct-116-cell.html [Content Brief]