GRK3 Antibody (YA7248)
(Synonyms: ADRBK2, BARK2, GRK3, G protein-coupled receptor kinase 3, Beta-adrenergic receptor kinase 2, Beta-ARK-2)GRK3 Antibody (YA7248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GRK3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IF-Tissue
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Reactivity :
Human
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
GRK3 Antibody (YA7248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GRK3.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 80 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 80 kDa
A synthesized peptide derived from human GRK3
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GRK3 is a GPCR kinase that phosphorylates agonist-occupied receptors and promotes β-arrestin-linked desensitization, internalization, and signaling control[1]. Mechanistically, GRK3 regulates receptor-specific pathways, including CXCR4 Ser-346/347 phosphorylation, where GRK3 knockdown produced the strongest reduction among GRK2, GRK3, and GRK6 knockdowns[2]. In disease models, GRK3 promoted prostate tumor growth and metastasis, regulated breast cancer migration and invasion, and drove gastric adenocarcinoma invasion through YAP1-associated transcriptional targets[3][4][5]. Compared with related isoforms, GRK3 differs from GRK2 in vivo because GRK2 loss causes embryonic lethality, whereas GRK3 deletion permits development but disrupts odorant receptor desensitization[6]. For experimental applications, LD2 specifically inhibited GRK3 but not the highly similar GRK2 in gastric adenocarcinoma models, while isochlorogenic acid A was identified as a GRK3 inhibitor in diabetic heart injury models[5][7].
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Subcellular Localization
Postsynapse,Presynapse
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Isoforms & Post-Translational Modification
P35626: 688 amino acids, molecular weight 79710 Da.
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Subunit
Interacts with GIT1
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SwissProt ID
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Synonyms
ADRBK2, BARK2, GRK3, G protein-coupled receptor kinase 3, Beta-adrenergic receptor kinase 2, Beta-ARK-2
Documentation
[1]. Belmonte SL, et al. G protein coupled receptor kinases as therapeutic targets in cardiovascular disease. Circ Res. 2011 Jul 22;109(3):309-19. [Content Brief]
[2]. Luo J, et al. G Protein-Coupled Receptor Kinase 3 and Protein Kinase C Phosphorylate the Distal C-Terminal Tail of the Chemokine Receptor CXCR4 and Mediate Recruitment of β-Arrestin. Mol Pharmacol. 2017 Jun;91(6):554-566. [Content Brief]
[3]. Li W, et al. GRK3 is essential for metastatic cells and promotes prostate tumor progression. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1521-6. [Content Brief]
[4]. Billard MJ, et al. G Protein Coupled Receptor Kinase 3 Regulates Breast Cancer Migration, Invasion, and Metastasis. PLoS One. 2016 Apr 6;11(4):e0152856. [Content Brief]
[5]. Li Y, et al. GRK3 is a poor prognosticator and serves as a therapeutic target in advanced gastric adenocarcinoma. J Exp Clin Cancer Res. 2022 Aug 23;41(1):257. [Content Brief]
[6]. Peppel K, et al. G protein-coupled receptor kinase 3 (GRK3) gene disruption leads to loss of odorant receptor desensitization. J Biol Chem. 1997 Oct 10;272(41):25425-8. [Content Brief]
[7]. Gao P, et al. G Protein-Coupled Receptor Kinase 3 Exacerbates Diabetic Heart Injuries Through Direct Phosphorylation of Cannabinoid Receptor 2 in Humans and Mice. Circulation. 2025 Sep 23;152(12):882-898. [Content Brief]
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