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Avi Tag Antibody (YA6862)

(Synonyms: AviTag; Avi; Avi Tag; GLNDIFEAQKIEWHE)
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Based on 1 Customer Validation

Avi Tag Antibody (YA6862) is a mouse-derived non-conjugated monoclonal antibody, targeting Avi Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, FC, ELISA, IF-Tissue

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Avi Tag Antibody (YA6862)
  • Avi Tag Antibody (YA6862) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:1000-5000 1:200-1000 1:200-1000 1:200-1000 1ug/Test 1:100-500 1:1000-5000

Product Details

Description

Avi Tag Antibody (YA6862) is a mouse-derived non-conjugated monoclonal antibody, targeting Avi Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
Immunogen

Recombinant Avi-Tag(GLNDIFEAQKIEWHE)

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Avi Tag Antibody (YA6862)
    Western blot analysis was performed by loading 0.1 μg (Lane 2), 0.5 μg (Lane 3), and 1 μg (Lane 4) of FGL2 Protein, Mouse (HEK293, His-Avi, Flag), 0.1 μg (Lane 5) and 0.5 μg (Lane 6) of Recombinant Tags Positive Control, C-His using Avi Tag antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Avi Tag is a The Avi-Tag protein is a short 15-amino acid peptide with a single biotinylated lysine residue, completely different from known naturally biotinylated sequences, and can be added to the N-terminus and C-terminus of the target protein.

  • Synonyms

    AviTag; Avi; Avi Tag; GLNDIFEAQKIEWHE

Avi Tag Antibody (YA6862) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed by loading 0.1 μg (Lane 2), 0.5 μg (Lane 3), and 1 μg (Lane 4) of FGL2 Protein, Mouse (HEK293, His-Avi, Flag), 0.1 μg (Lane 5) and 0.5 μg (Lane 6) of Recombinant Tags Positive Control, C-His using Avi Tag antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Avi Tag Antibody (YA6862) Product Image
    1/16
  • Experimental Validation Results for Avi Tag Antibody (YA6862)
  • Avi Tag Antibody (YA6862) Product Image
  • Experimental Validation Results for Avi Tag Antibody (YA6862)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed by loading 0.1 μg (Lane 2), 0.5 μg (Lane 3), and 1 μg (Lane 4) of FGL2 Protein, Mouse (HEK293, His-Avi, Flag), 0.1 μg (Lane 5) and 0.5 μg (Lane 6) of Recombinant Tags Positive Control, C-His using Avi Tag antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Avi Tag Antibody (YA6862)
  • Avi Tag Antibody (YA6862) Product Image
    MedChemExpress Validation

    Western blot analysis was performed by loading 0.1 μg (Lane 2), 0.5 μg (Lane 3), and 1 μg (Lane 4) of FGL2 Protein, Mouse (HEK293, His-Avi, Flag), 0.1 μg (Lane 5) and 0.5 μg (Lane 6) of Recombinant Tags Positive Control, C-His using Avi Tag antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Avi Tag Antibody (YA6862) Product Image WB