DPP8 Antibody (YA7861)(PBS only)

Cat. No.: HY-P88177A: Data Sheet User Guide for Antibodies
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DPP8 Antibody (YA7861) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DPP8.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DPP8 Antibody (YA7861) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DPP8.

Host

Mouse

Molecular Weight

Predicted band size: 101.2 kDa

Species Reactivity

Human, Dog, Rat, Mouse

SwissProt ID

Q6V1X1

Immunogen

Full length human recombinant protein of human DPP8 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 (PubMed:11012666, PubMed:12534281, PubMed:12662155, PubMed:15039077, PubMed:15664838, PubMed:20536396, PubMed:29382749). Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8 (PubMed:27820798, PubMed:29967349, PubMed:32796818). Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation (PubMed:33731929, PubMed:33731932, PubMed:34019797). The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain
Induction: In activated T-cells

Isoforms & Post-Translational Modification：Q6V1X1 has 6 isomers: Q6V1X1-1: 103358 Da (predicted); Q6V1X1-2: 97464 Da (predicted); Q6V1X1-3: 101422 Da (predicted); Q6V1X1-4: 89908 Da (predicted); Q6V1X1-5: 97739 Da (predicted); Q6V1X1-6: 83398 Da (predicted).

Subunit：Homodimer (PubMed:20536396, PubMed:29382749)

Synonyms

DP8; DPRP1; MSTP141

Documentation

User Guide for Antibodies (1077 KB)