Endophilin B1 Antibody (YA9310)
(Synonyms: KIAA0491, CGI-61, SH3GLB1, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, Bif-1)Endophilin B1 Antibody (YA9310) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Endophilin B1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
Endophilin B1 Antibody (YA9310) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Endophilin B1.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 43 kDa
OMIM: 609287
A synthesized peptide derived from human Endophilin B1.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
Endophilin B1 may be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion. Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation. Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2. Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity)[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Golgi apparatus membrane; Mitochondrion outer membrane; Cytoplasmic vesicle, autophagosome membrane; Midbody
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Expression
Tissue_Specificity: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. -
Isoforms & Post-Translational Modification
Endophilin B1 has 3 isoforms, Q9Y371-1: amino acid length is 365, molecular weight is 40796 Da (predicted); Q9Y371-2: amino acid length is 386, molecular weight is 43196 Da (predicted); Q9Y371-3: amino acid length is 265, molecular weight is 29318 Da (predicted).
Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization -
Subunit
Homodimer, and heterodimer with SH3GLB2.
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SwissProt ID
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Synonyms
KIAA0491, CGI-61, SH3GLB1, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, Bif-1
Documentation
References
[1]. Karbowski M, et al. Endophilin B1 is required for the maintenance of mitochondrial morphology. J Cell Biol. 2004 Sep 27;166(7):1027-39. [Content Brief]
[2]. Farsad K, et al. Generation of high curvature membranes mediated by direct endophilin bilayer interactions. J Cell Biol. 2001 Oct 15;155(2):193-200. [Content Brief]
[3]. Takahashi Y, et al. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol Cell Biol. 2005 Nov;25(21):9369-82. [Content Brief]
[4]. Takahashi Y, et al. Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis. Nat Cell Biol. 2007 Oct;9(10):1142-51. [Content Brief]
[5]. Takahashi Y, et al. Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy. 2011 Jan;7(1):61-73. [Content Brief]
[6]. Thoresen SB, et al. A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic. Exp Cell Res. 2010 Dec 10;316(20):3368-78. [Content Brief]
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