MAP2 Antibody (YA5826)

Cat. No.: HY-P86134: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

MAP2 Antibody (YA5826) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAP2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

MAP2 Antibody (YA5826) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAP2.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 200 kDa;

Observed band size: 280 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P11137

Gene ID

4133 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG/Kappa

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Neuro-2a (lane 2(20μg), SH-SY5Y (lane 3(20μg), THP-1 (lane 4(20μg), C6 (lane 5(20μg) using MAP2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules

Subcellular Localization：Cytoplasm, cytoskeleton; Cell projection, dendrite

Isoforms & Post-Translational Modification：P11137 has 4 isomers: P11137-1: 199526 Da (predicted); P11137-2: 49640 Da (predicted); P11137-3: 199084 Da (predicted); P11137-4: 58954 Da (predicted).
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity)

Subunit：Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites. Interacts with DPYSL5 (PubMed:33894126)

Synonyms

MAP2; Microtubule-associated protein 2; MAP-2

Documentation

Select Batch:

Data Sheet (233 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)