TORC1 Antibody (YA5170)

Cat. No.: HY-P85478: Data Sheet User Guide for Antibodies
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TORC1 Antibody (YA5170) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TORC1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TORC1 Antibody (YA5170) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TORC1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 67 kDa;

Species Reactivity

Human, Mouse, Rat, Pig

SwissProt ID

Q6UUV9

Gene ID

23373 [NCBI]

Immunogen

Purified recombinant human TORC1 (N-terminus) protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
FC FC: Flow Cytometry	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock; (Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is highly expressed in the human brain, localized to specific regions such as the prefrontal cortex and cerebellum. It is expressed at very low levels in other tissues, such as the heart, spleen, lungs, skeletal muscle, salivary glands, ovaries, and kidneys.

Isoforms & Post-Translational Modification：Q6UUV9 has 3 isomers: Q6UUV9-1: 67300 Da (predicted); Q6UUV9-2: 68782 Da (predicted); Q6UUV9-3: 62530 Da (predicted).
Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1 (By similarity)

Subunit：Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 (PubMed:14536081). 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction (PubMed:14536081). Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1 (PubMed:14536081). Interacts with 14-3-3 proteins, including YWHAE/14-3-3 epsilon (PubMed:30611118). Interacts with calmodulin-dependent catalytic subunit PPP3CA/calcineurin A (PubMed:30611118)

Synonyms

CRTC1; KIAA0616; MECT1; TORC1; WAMTP1; CREB-regulated transcription coactivator 1; Mucoepidermoid carcinoma translocated protein 1; Transducer of regulated cAMP response element-binding protein 1; TORC-1; Transducer of CREB protein 1

Documentation

User Guide for Antibodies (1077 KB)