Nanog Antibody (YA4984)

Cat. No.: HY-P85292: Data Sheet User Guide for Antibodies
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Nanog Antibody (YA4984) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Nanog.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Nanog Antibody (YA4984) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Nanog.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 34 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9H9S0

Gene ID

79923 [NCBI]

Immunogen

Recombinant human Nanog protein (1-100aa). The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-800
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-800
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-800

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is expressed (at the protein level) in testicular cancer and its derived germ cell tumors. It is also expressed in fetal gonads, ovaries, and testes. Additionally, it is expressed in ovarian teratoma cell lines and testicular embryonal carcinoma. It is not expressed in many somatic organs and oocytes.

Isoforms & Post-Translational Modification：Q9H9S0 has 2 isomers: Q9H9S0-1: 34620 Da (predicted); Q9H9S0-2: 32838 Da (predicted).

Subunit：Interacts with SMAD1 (By similarity). Interacts with SALL4 (By similarity). Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity). Interacts with NSD2 (By similarity)

Synonyms

NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog

Documentation

User Guide for Antibodies (1077 KB)