Nanog Antibody (YA4984)
(Synonyms: NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog)Nanog Antibody (YA4984) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Nanog.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB; IHC-P; IHC-F; ICC/IF; IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-800 | 1:50-800 | 1:50-200 | 1:50-800 |
Product Details
Nanog Antibody (YA4984) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Nanog.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 34 kDa;
Recombinant human Nanog protein (1-100aa). The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Nanog transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation[1].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:It is expressed (at the protein level) in testicular cancer and its derived germ cell tumors. It is also expressed in fetal gonads, ovaries, and testes. Additionally, it is expressed in ovarian teratoma cell lines and testicular embryonal carcinoma. It is not expressed in many somatic organs and oocytes. -
Isoforms & Post-Translational Modification
Q9H9S0 has 2 isomers: Q9H9S0-1: 34620 Da (predicted); Q9H9S0-2: 32838 Da (predicted).
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Subunit
Interacts with SMAD1 (By similarity). Interacts with SALL4 (By similarity). Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity). Interacts with NSD2 (By similarity)
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SwissProt ID
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Synonyms
NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog
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