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Doublecortin Antibody (YA474)

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Based on 1 Customer Validation

Doublecortin Antibody (YA474) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Doublecortin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Doublecortin Antibody (YA474)
  • Experimental Validation Results for Doublecortin Antibody (YA474)
  • Doublecortin Antibody (YA474) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:50-1:100

Product Details

Description

Doublecortin Antibody (YA474) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Doublecortin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 41/45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Doublecortin.AA range:22-71.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102078

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Doublecortin Antibody (YA474)
    Western blot analysis of extracts from Neuro-2a (lane 2(20μg) , Neuro-2a (lane 3(40μg),using Doublecortin Antibody (HY-P80108). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Doublecortin Antibody (YA474)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Doublecortin Antibody(HY-P80108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration

  • Subcellular Localization

    Cytoplasm; Cell projection, neuron projection

  • Expression


    Tissue_specificity:It is highly expressed in fetal brain neurons (most cells in the cortical plate, intermediate zone, and ventricular zone) , but not in other fetal tissues. In humans, it is highly expressed in the frontal lobe of the brain, but very low in other brain regions, and is not detected in the heart, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas.

  • Subunit

    Interacts with tubulin (PubMed:27292316). Interacts with USP9X (PubMed:24607389)

  • SwissProt ID

    O43602

  • Gene ID
    1641 [NCBI]
  • Research Field

    Cell Biology

Doublecortin Antibody (YA474) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Neuro-2a (lane 2(20μg) , Neuro-2a (lane 3(40μg),using Doublecortin Antibody (HY-P80108). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Doublecortin Antibody(HY-P80108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Doublecortin Antibody (YA474) Product Image
    1/16
  • Experimental Validation Results for Doublecortin Antibody (YA474)
  • Experimental Validation Results for Doublecortin Antibody (YA474)
  • Doublecortin Antibody (YA474) Product Image
  • Experimental Validation Results for Doublecortin Antibody (YA474)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Neuro-2a (lane 2(20μg) , Neuro-2a (lane 3(40μg),using Doublecortin Antibody (HY-P80108). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Doublecortin Antibody (YA474)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Doublecortin Antibody(HY-P80108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Doublecortin Antibody (YA474)
  • Experimental Validation Results for Doublecortin Antibody (YA474)
  • Doublecortin Antibody (YA474) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Neuro-2a (lane 2(20μg) , Neuro-2a (lane 3(40μg),using Doublecortin Antibody (HY-P80108). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Doublecortin Antibody (YA474) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Doublecortin Antibody(HY-P80108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Doublecortin Antibody (YA474) Product Image WB
  • Doublecortin Antibody (YA474) Product Image FC