Doublecortin Antibody (YA474)

Cat. No.: HY-P80108: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
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Doublecortin Antibody (YA474) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Doublecortin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Doublecortin Antibody (YA474) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Doublecortin Antibody (YA474) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Doublecortin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 41 kDa;

Observed band size: 41/45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43602

Gene ID

1641 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Doublecortin.AA range:22-71.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB FC

Western blot analysis of extracts from Neuro-2a (lane 2(20μg) , Neuro-2a (lane 3(40μg),using Doublecortin Antibody (HY-P80108). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10⁶ SH-SY5Y cells labeling Doublecortin Antibody(HY-P80108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration

Subcellular Localization：Cytoplasm; Cell projection, neuron projection

Expression：
Tissue_specificity:It is highly expressed in fetal brain neurons (most cells in the cortical plate, intermediate zone, and ventricular zone) , but not in other fetal tissues. In humans, it is highly expressed in the frontal lobe of the brain, but very low in other brain regions, and is not detected in the heart, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas.

Subunit：Interacts with tubulin (PubMed:27292316). Interacts with USP9X (PubMed:24607389)

RRID

AB_3102078

Database

Entrez Gene: 1641 Human ; 13193 Mouse ;

SwissProt: O43602 Human ; O88809 Mouse ; Q9ESI7 Rat

OMIM: 300067 Human

Research Field

Cell Biology

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Doublecortin Antibody (YA474) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Doublecortin Antibody (YA474)

Doublecortin Antibody (YA474) Featured Recommendations:

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Background

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Doublecortin Antibody (YA474) Related Classifications

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