RAP80 Antibody (YA3208)(PBS only)

Cat. No.: HY-P83463A: Data Sheet User Guide for Antibodies
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RAP80 Antibody (YA3208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP80.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RAP80 Antibody (YA3208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP80.

Host

Rabbit

Molecular Weight

Predicted band size: 80 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96RL1

Gene ID

51720 [NCBI]

Immunogen

A synthetic peptide of human RAP80

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.38). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is expressed in the testes, ovaries, thymus, and heart. It is also expressed in testicular germ cells.

Isoforms & Post-Translational Modification：Q96RL1 has 5 isomers: Q96RL1-1: 79727 Da (predicted); Q96RL1-2: 61325 Da (predicted); Q96RL1-3: 70744 Da (predicted); Q96RL1-4: 38477 Da (predicted); Q96RL1-5: 17412 Da (predicted).
Sumoylated;Phosphorylated upon DNA damage by ATM or ATR

Subunit：Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1 (PubMed:24075985).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

RAP80; X2HRIP110

Documentation

User Guide for Antibodies (1077 KB)