TOM1 Antibody (YA6807)
(Synonyms: FLJ33404 antibody; OTTHUMP00000028777 antibody; Target of myb 1 antibody; Target of Myb protein 1 antibody; Target of myb1 antibody; TOM 1 antibody; TOM1 antibody; TOM1_HUMAN antibody; )TOM1 Antibody (YA6807) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TOM1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IF-Tissue, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|
| Dilution Ratio | 1:2000 | 1:200 | 1:200-1:1000 |
Product Details
TOM1 Antibody (YA6807) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TOM1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 54 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 54 kDa
Recombinant protein within human TOM1 aa 300-450.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TOM1 is an Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pathways. Acts as a MYO6/Myosin VI adapter protein that targets MYO6 to endocytic structures. Together with MYO6, required for autophagosomal delivery of endocytic cargo, the maturation of autophagosomes and their fusion with lysosomes. MYO6 links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN. Binds to polyubiquitinated proteins via its GAT domain. In a complex with TOLLIP, recruits ubiquitin-conjugated proteins onto early endosomes. The Tom1-Tollip complex may regulate endosomal trafficking by linking polyubiquitinated proteins to clathrin. Mediates clathrin recruitment to early endosomes by ZFYVE16. Modulates binding of TOLLIP to phosphatidylinositol 3-phosphate (PtdIns(3)P) via binding competition; the association with TOLLIP may favor the release of TOLLIP from endosomal membranes, allowing TOLLIP to commit to cargo trafficking. Acts as a phosphatidylinositol 5-phosphate (PtdIns(5)P) effector by binding to PtdIns(5)P, thereby regulating endosomal maturation. PtdIns(5)P-dependent recruitment to signaling endosomes may block endosomal maturation. Also inhibits Toll-like receptor (TLR) signaling and participates in immune receptor recycling[1][2][3][4][5][6][7].
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Subcellular Localization
Cytoplasm,Endosome membrane,Early endosome membrane
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Expression
Tissue_Specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver -
Isoforms & Post-Translational Modification
O60784 has four isomers: O60784-1: 53818 Da (predicted); O60784-2: 53875 Da (predicted); O60784-3: 48673 Da (predicted); O60784-4: 50269 Da (predicted).
Monoubiquitinated -
Subunit
Found in a complex with TOLLIP; interacts (via GAT domain) with TOLLIP (via N-terminus); the interactions leads to TOM1-recruitment to endosomes and inhibition of TOLLIP binding to PtdIns(3)P (PubMed:14563850, PubMed:15047686, PubMed:26320582, PubMed:31263572)
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SwissProt ID
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Synonyms
FLJ33404 antibody; OTTHUMP00000028777 antibody; Target of myb 1 antibody; Target of Myb protein 1 antibody; Target of myb1 antibody; TOM 1 antibody; TOM1 antibody; TOM1_HUMAN antibody;
Documentation
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