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Phospho-Cyclin B1 (Ser126) Antibody (YA208)

(Synonyms: CCNB1; CCNB; G2/mitotic-specific cyclin-B1)
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Based on 1 Customer Validation

Phospho-Cyclin B1 (Ser126) Antibody (YA208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Cyclin B1 (Ser126).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-Cyclin B1 (Ser126) Antibody (YA208)
  • Phospho-Cyclin B1 (Ser126) Antibody (YA208) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Phospho-Cyclin B1 (Ser126) Antibody (YA208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Cyclin B1 (Ser126).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Species Reactivity Database

Entrez Gene: 891 Human

SwissProt: P14635 Human

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser126 of Human Cyclin B1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102719

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Cyclin B1 (Ser126) Antibody (YA208)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Phospho-Cyclin B1 (Ser126) (HY-P80806) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Isoforms & Post-Translational Modification

    P14635 has 2 isomers: P14635-1: 48337 Da (predicted); P14635-2: 44102 Da (predicted).
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811);Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site

  • Subunit

    Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3 (PubMed:15790566). Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1 (PubMed:21540187)

  • SwissProt ID

    P14635

  • Gene ID
    891 [NCBI]
  • Synonyms

    CCNB1; CCNB; G2/mitotic-specific cyclin-B1

  • Research Field

    Cell Biology

Phospho-Cyclin B1 (Ser126) Antibody (YA208) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Phospho-Cyclin B1 (Ser126) (HY-P80806) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Cyclin B1 (Ser126) Antibody (YA208) Product Image
    1/16
  • Experimental Validation Results for Phospho-Cyclin B1 (Ser126) Antibody (YA208)
  • Phospho-Cyclin B1 (Ser126) Antibody (YA208) Product Image
  • Experimental Validation Results for Phospho-Cyclin B1 (Ser126) Antibody (YA208)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Phospho-Cyclin B1 (Ser126) (HY-P80806) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Cyclin B1 (Ser126) Antibody (YA208)
  • Phospho-Cyclin B1 (Ser126) Antibody (YA208) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using Phospho-Cyclin B1 (Ser126) (HY-P80806) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Cyclin B1 (Ser126) Antibody (YA208) Product Image WB