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Phostensin Antibody (YA1074)

(Synonyms: HKMT1098; KIAA1949.)
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Based on 1 Customer Validation

Phostensin Antibody (YA1074) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phostensin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phostensin Antibody (YA1074)
  • Phostensin Antibody (YA1074) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Phostensin Antibody (YA1074) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phostensin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 110，26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database

Entrez Gene: 170954 Human

SwissProt: Q6NYC8 Human

Immunogen

Purified recombinant human Phostensin protein fragments expressed in E.coli.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103445

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phostensin Antibody (YA1074)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Jurkat (lane 3, 15 μg), Raji (lane 4, 15 μg), Ramos (lane 5, 15 μg), and MDA-MB-435S (lane 6, 15 μg) using Phostensin Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Phostensin may target protein phosphatase 1 to F-actin cytoskeleton; May target protein phosphatase 1 to F-actin cytoskeleton

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Isomer 4 is mainly expressed in leukocytes and spleen.

  • Isoforms & Post-Translational Modification

    Q6NYC8 has 4 isomers: Q6NYC8-1: 67943 Da (predicted); Q6NYC8-2: 42833 Da (predicted); Q6NYC8-3: 39373 Da (predicted); Q6NYC8-4: 17771 Da (predicted).

  • Subunit

    Interacts with Protein phosphatase 1 (PP1)

  • SwissProt ID

    Q6NYC8

  • Gene ID
    170954 [NCBI]
  • Synonyms

    HKMT1098; KIAA1949.

  • Research Field

    Signal Transduction

Phostensin Antibody (YA1074) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Jurkat (lane 3, 15 μg), Raji (lane 4, 15 μg), Ramos (lane 5, 15 μg), and MDA-MB-435S (lane 6, 15 μg) using Phostensin Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Phostensin Antibody (YA1074) Product Image
    1/16
  • Experimental Validation Results for Phostensin Antibody (YA1074)
  • Phostensin Antibody (YA1074) Product Image
  • Experimental Validation Results for Phostensin Antibody (YA1074)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Jurkat (lane 3, 15 μg), Raji (lane 4, 15 μg), Ramos (lane 5, 15 μg), and MDA-MB-435S (lane 6, 15 μg) using Phostensin Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Phostensin Antibody (YA1074)
  • Phostensin Antibody (YA1074) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Jurkat (lane 3, 15 μg), Raji (lane 4, 15 μg), Ramos (lane 5, 15 μg), and MDA-MB-435S (lane 6, 15 μg) using Phostensin Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:10000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Phostensin Antibody (YA1074) Product Image WB