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MMP-1 Antibody (YA3394)

(Synonyms: MMP1; CLG; Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1)
Customer Review

Based on 1 Customer Validation

MMP-1 Antibody (YA3394) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MMP-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC, ICC/IF, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL IHC-P ICC
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • MMP-1 Antibody (YA3394) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

MMP-1 Antibody (YA3394) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MMP-1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database

Entrez Gene: 4312 Human

SwissProt: P03956 Human

Immunogen

A synthesized peptide derived from human MMP1 aa110-145.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105039

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunocytochemistry analysis of HepG2 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    Immunocytochemistry analysis of MCF-7 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase or collagenase-1, is a zinc-dependent extracellular matrix protease that cleaves fibrillar collagens, particularly types I, II, and III collagen[1][2]. MMP-1 initiates collagenolysis by disrupting the triple-helical structure of collagen, enabling subsequent extracellular matrix remodeling during tissue repair and pathological processes[2][3]. Mechanistically, MMP-1 activity is regulated at transcriptional and enzymatic levels, including control by signaling pathways such as AP-1-associated transcriptional regulation[4]. In disease models, increased MMP-1 expression contributes to extracellular matrix degradation in rheumatoid arthritis, tumor invasion, metastasis, and other destructive tissue remodeling conditions[1][4]. MMP-1 promotes keratinocyte migration by enabling movement across type I collagen matrices, demonstrating its role in wound repair models[3]. Compared with related collagenases such as MMP-8 and MMP-13, MMP-1 is broadly expressed by multiple cell types and preferentially functions as an interstitial collagenase involved in degradation of stromal collagens[1][2]. Unlike membrane-type MMPs such as MT1-MMP, MMP-1 is a secreted collagenase that primarily acts within extracellular matrix environments[1][5]. For experimental applications, MMP-1 activity is commonly investigated using genetic regulation studies, collagen degradation assays, and pharmacological inhibition approaches with broad-spectrum or selective metalloproteinase inhibitors[3][6]. MMP-1 inhibition strategies have been explored to study extracellular matrix remodeling mechanisms, although clinical translation of MMP inhibitors remains limited by specificity and safety challenges[2].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Subunit

    (Microbial infection) Interacts with HIV-1 Tat

  • SwissProt ID

    P03956

  • Gene ID
    4312 [NCBI]
  • Synonyms

    MMP1; CLG; Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1

  • Research Field

    Primary Antibody; Rabbit Monoclonal Antibody

References

[1]. Brinckerhoff CE, et al. Matrix metalloproteinases: a tail of a frog that became a prince. Nature Reviews Molecular Cell Biology. 2002;3(3):207-214. [Content Brief]

[2]. Amar S, et al. Matrix metalloproteinase collagenolysis in health and disease. Biochim Biophys Acta Mol Cell Res. 2017 Nov;1864(11 Pt A):1940-1951. [Content Brief]

[3]. Pilcher BK, et al. The Activity of Collagenase-1 Is Required for Keratinocyte Migration on a Type I Collagen Matrix. Journal of Cell Biology. 1997;137(6):1445-1457. [Content Brief]

[4]. Sun Y, et al. p53 down-regulates human matrix metalloproteinase-1 (Collagenase-1) gene expression. Journal of Biological Chemistry. 1999;274(17):11535-11540. [Content Brief]

[5]. Nagase H, et al. Matrix metalloproteinases. Journal of Biological Chemistry. 1999;274(31):21491-21494. [Content Brief]

[6]. Pardo A, et al. MMP-1: the elder of the family. International Journal of Biochemistry Cell Biology. 2005;37(2):283-288. [Content Brief]

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MMP-1 Antibody (YA3394) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MMP-1 Antibody (YA3394) Product Image
    1/16
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • MMP-1 Antibody (YA3394) Product Image
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • Experimental Validation Results for MMP-1 Antibody (YA3394)
  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using MMP-1 Antibody (YA3394). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80754A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MMP-1 Antibody (YA3394) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling MMP-1 with MMP-1 Antibody (HY-P80754A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MMP-1 Antibody (HY-P80754A) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MMP-1 Antibody (YA3394) Product Image IHC-P
  • MMP-1 Antibody (YA3394) Product Image IHC-P
  • MMP-1 Antibody (YA3394) Product Image IHC-P
  • MMP-1 Antibody (YA3394) Product Image IHC-P
  • MMP-1 Antibody (YA3394) Product Image IHC-P
  • MMP-1 Antibody (YA3394) Product Image IHC-P
  • MMP-1 Antibody (YA3394) Product Image ICC
  • MMP-1 Antibody (YA3394) Product Image ICC