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Calumenin Antibody (YA813)

(Synonyms: CALU; CALU_HUMAN; Calumenin 1; Calumenin 2; Calumenin; Crocalbin; crocalbin-like protein; FLJ90608; IEF SSP 9302; multiple EF-hand protein; OTTHUMP00000212712; OTTHUMP00000212713; OTTHUMP00000212714; OTTHUMP00000212715.)
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Based on 1 Customer Validation

Calumenin Antibody (YA813) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Calumenin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Calumenin Antibody (YA813)
  • Calumenin Antibody (YA813) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Calumenin Antibody (YA813) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Calumenin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Calumenin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102917

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Calumenin Antibody (YA813)
    Western blot analysis of extracts from A431 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and HEK293 (lane5(20μg) using Calumenin Antibody (HY-P80579). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Calumenin is involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity

  • Subcellular Localization

    Endoplasmic reticulum membrane; Golgi apparatus; Secreted; Melanosome; Sarcoplasmic reticulum lumen

  • Expression


    Tissue_specificity:It is widely expressed. It is highly expressed in the heart, placenta, and skeletal muscle, lower in the lungs, kidneys, and pancreas, and extremely low in the brain and liver.

  • Isoforms & Post-Translational Modification

    O43852 has 15 isomers: O43852-1: 37107 Da (predicted); O43852-2: 37135 Da (predicted); O43852-3: 38051 Da (predicted); O43852-4: 38079 Da (predicted); O43852-5: 26892 Da (predicted); O43852-6: 30957 Da (predicted); O43852-7: 18789 Da (predicted); O43852-8: 23519 Da (predicted); O43852-9: 17639 Da (predicted); O43852-10: 26326 Da (predicted); O43852-11: 19993 Da (predicted); O43852-12: 8511 Da (predicted); O43852-13: 16291 Da (predicted); O43852-14: 17235 Da (predicted); O43852-15: 19455 Da (predicted).

  • Subunit

    Interacts with GGCX

  • SwissProt ID

    O43852

  • Gene ID
    813 [NCBI]
  • Synonyms

    CALU; CALU_HUMAN; Calumenin 1; Calumenin 2; Calumenin; Crocalbin; crocalbin-like protein; FLJ90608; IEF SSP 9302; multiple EF-hand protein; OTTHUMP00000212712; OTTHUMP00000212713; OTTHUMP00000212714; OTTHUMP00000212715.

  • Research Field

    Tags & Cell Markers

Calumenin Antibody (YA813) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and HEK293 (lane5(20μg) using Calumenin Antibody (HY-P80579). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Calumenin Antibody (YA813) Product Image
    1/16
  • Experimental Validation Results for Calumenin Antibody (YA813)
  • Calumenin Antibody (YA813) Product Image
  • Experimental Validation Results for Calumenin Antibody (YA813)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and HEK293 (lane5(20μg) using Calumenin Antibody (HY-P80579). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Calumenin Antibody (YA813)
  • Calumenin Antibody (YA813) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane2(20μg), K562 (lane3(20μg), Hela (lane4(20μg) and HEK293 (lane5(20μg) using Calumenin Antibody (HY-P80579). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Calumenin Antibody (YA813) Product Image WB