2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. BAG1 Antibody (YA932)

BAG1 Antibody (YA932) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BAG1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

BAG1 Antibody (YA932) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BAG1.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 39 kDa;
Observed band size: 52,46,33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Purified recombinant human Bag1 protein fragments expressed in E.coli.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Transfected Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:24318877, PubMed:27474739, PubMed:9873016). Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed:12724406). Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed:9305631). Involved in the STUB1-mediated proteasomal degradation of ESR1 in response to age-related circulating estradiol (17-beta-estradiol/E2) decline, thereby promotes neuronal apoptosis in response to ischemic reperfusion injury (By similarity)
Subcellular Localization：Nucleus; Cytoplasm; Cytoplasm; Nucleus; Cytoplasm; Nucleus
Expression：
Tissue_specificity:Isomer 4 is the most abundant isomer. It is universally expressed in most tissues except the liver, colon, breast, and myometrium. Isomer 1 is expressed in the ovary and testis. Isomer 4 is expressed in various tumor cell lines and is persistently highly expressed in leukemia and lymphoma cell lines. Isomer 1 is expressed in prostate cancer, breast cancer, and leukemia cell lines. Isomer 3 is the least abundant isomer (at the protein level) in tumor cell lines.

Induction:Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium
Isoforms & Post-Translational Modification：Q99933 has 4 isomers: Q99933-1: 38779 Da (predicted); Q99933-2: 34866 Da (predicted); Q99933-3: 31117 Da (predicted); Q99933-4: 25989 Da (predicted).
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation
Subunit：Homodimer. Forms a heteromeric complex with HSP70/HSC70 (PubMed:9305631). Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70 (PubMed:24318877, PubMed:27474739, PubMed:9305631, PubMed:9679980). Interacts with NR3C1 (PubMed:10477749). Interacts with the N-terminal region of MAPRE2 (PubMed:15986447). Interacts with PPP1R15A (PubMed:12724406). Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2 (PubMed:9305631). Interacts with SIAH1 (PubMed:9582267). Interacts with HSPA8 (via NBD) (PubMed:24318877, PubMed:27474739). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD) (PubMed:24318877). Interacts with SIAH2 (By similarity). Interacts with ESR1; the interaction is promoted in the absence of estradiol (17-beta-estradiol/E2) (By similarity)
Database

Entrez Gene: 573 Human ; 12017 Mouse ; 297994 Rat

SwissProt: Q99933 Human ; Q60739 Mouse ; B0K019 Rat

OMIM: 601497 Human

Research Field

Cell Biology
Synonyms
BAG1; HAP; BAG family molecular chaperone regulator 1; BAG-1; Bcl-2-associated athanogene 1
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

BAG1 Antibody (YA932) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BAG1 Antibody (YA932)
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