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FOXL2 Antibody (YA6874)

(Synonyms: Blepharophimosis antibody; BPES 1 antibody; Forkhead box protein L2 antibody)
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Based on 1 Customer Validation

FOXL2 Antibody (YA6874) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXL2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
  • FOXL2 Antibody (YA6874) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:1000

Product Details

Description

FOXL2 Antibody (YA6874) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXL2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Immunogen

Synthetic peptide within Human FOXL2 aa 1-50 / 376.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719285

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FOXL2 Antibody (YA6874)
    Western blot analysis of extracts from K562 (lane 1(20μg)) and THP-1 (lane 2(20μg)) using FOXL2 Antibody (HY-P87186) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
    Western blot analysis was performed on nuclear protein extracts (35 μg) from K562 (lane 2), THP-1 (lane 3), and HeLa (lane 4) using FOXL2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Histone H3 Antibody (HY-P86672) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    FOXL2 transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Is a regulator of CYP19 expression. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element. Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_Specificity: In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary
    Induction: In granulosa-like cells, up-regulated at transcript and protein levels under oxidative stress and heat-shock conditions. Down-regulated by SIRT1

  • Isoforms & Post-Translational Modification

    P58012: 376 amino acids, molecular weight 38772 Da.

  • Subunit

    Interacts with ESR1 (By similarity)

  • SwissProt ID

    P58012

  • Synonyms

    Blepharophimosis antibody; BPES 1 antibody; Forkhead box protein L2 antibody

FOXL2 Antibody (YA6874) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) and THP-1 (lane 2(20μg)) using FOXL2 Antibody (HY-P87186) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on nuclear protein extracts (35 μg) from K562 (lane 2), THP-1 (lane 3), and HeLa (lane 4) using FOXL2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Histone H3 Antibody (HY-P86672) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FOXL2 Antibody (YA6874) Product Image
    1/16
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
  • FOXL2 Antibody (YA6874) Product Image
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) and THP-1 (lane 2(20μg)) using FOXL2 Antibody (HY-P87186) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on nuclear protein extracts (35 μg) from K562 (lane 2), THP-1 (lane 3), and HeLa (lane 4) using FOXL2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Histone H3 Antibody (HY-P86672) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
  • Experimental Validation Results for FOXL2 Antibody (YA6874)
  • FOXL2 Antibody (YA6874) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 1(20μg)) and THP-1 (lane 2(20μg)) using FOXL2 Antibody (HY-P87186) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • FOXL2 Antibody (YA6874) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on nuclear protein extracts (35 μg) from K562 (lane 2), THP-1 (lane 3), and HeLa (lane 4) using FOXL2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Histone H3 Antibody (HY-P86672) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FOXL2 Antibody (YA6874) Product Image WB
  • FOXL2 Antibody (YA6874) Product Image WB