SMAD7 Antibody (YA7206)

Cat. No.: HY-P87522: Ficha de datos User Guide for Antibodies
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SMAD7 Antibody is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to SMAD7.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño	Stock	Cantidad
20 μL	Obtener un presupuesto 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Obtener un presupuesto 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Obtener un presupuesto 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

SMAD7 Antibody is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to SMAD7.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 46 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O15105

Immunogen

KLH conjugated synthetic peptide derived from human SMAD7.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:200-1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IP IP: Immunoprecipitation	1-2μg per 100-500μg Total protein
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:50-3000

Sensitivity	Endogenous	Pureza	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Envío

Shipping with blue ice.

Background

Function：Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed:21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator

Subcellular Localization：Nucleus,Cytoplasm

Expression：
Tissue_Specificity: Ubiquitous with higher expression in the lung and vascular endothelium
Induction: By TGFB1

Isoforms & Post-Translational Modification：O15105 has three isomers: O15105-1: 46426 Da (predicted); O15105-2: 23901 Da (predicted); O15105-3: 46355 Da (predicted).
Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity)丨Ubiquitinated by WWP1 (By similarity)丨Acetylation prevents ubiquitination and degradation mediated by SMURF1

Subunit：Interacts with WWP1 (By similarity)

Synonyms

Mad homolog 7, hSMAD7, CRCS3, SMAD family member 7,

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/colo-205-cell.html [Content Brief]