SMAD7 Antibody (YA7206)
(Synonyms: Mad homolog 7, hSMAD7, CRCS3, SMAD family member 7, )
Based on 1 Customer Validation
SMAD7 Antibody is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to SMAD7.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
WB, IHC-P, ICC/IF, IP, ELISA, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:50-500 | 1:50-500 | 1:100-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
SMAD7 Antibody is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to SMAD7.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 46 kDa
KLH conjugated synthetic peptide derived from human SMAD7.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
SMAD7 is an Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator[1].
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Subcellular Localization
Nucleus,Cytoplasm
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Expression
Tissue_Specificity: Ubiquitous with higher expression in the lung and vascular endothelium
Induction: By TGFB1 -
Isoforms & Post-Translational Modification
O15105 has three isomers: O15105-1: 46426 Da (predicted); O15105-2: 23901 Da (predicted); O15105-3: 46355 Da (predicted).
Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity)丨Ubiquitinated by WWP1 (By similarity)丨Acetylation prevents ubiquitination and degradation mediated by SMURF1 -
Subunit
Interacts with WWP1 (By similarity)
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SwissProt ID
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Synonyms
Mad homolog 7, hSMAD7, CRCS3, SMAD family member 7,
Documentation
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