PKMYT1 Antibody (YA8105)

Cat. No.: HY-P88421: Data Sheet COA
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User Guide for Antibodies
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PKMYT1 Antibody (YA8105) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PKMYT1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PKMYT1 Antibody (YA8105) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PKMYT1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 54.3 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q99640

Immunogen

Full length human recombinant protein of human PKMYT1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-250
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins (PubMed:10373560, PubMed:10504341, PubMed:9001210, PubMed:9268380). Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation (PubMed:9001210, PubMed:9268380). May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect (PubMed:9001210, PubMed:9268380)

Subcellular Localization：Endoplasmic reticulum membrane,Golgi apparatus membrane

Isoforms & Post-Translational Modification：Q99640 has four isomers: Q99640-1: 54521 Da (predicted); Q99640-2: 52146 Da (predicted); Q99640-3: 53542 Da (predicted); Q99640-4: 47284 Da (predicted).
Autophosphorylated

Subunit：Interacts with CDC2-CCNB1 complex

Synonyms

MYT1; PPP1R126

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)