Mucin 4 Antibody (YA3264)(PBS only)

Cat. No.: HY-P83519A: Data Sheet User Guide for Antibodies
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Mucin 4 Antibody (YA3264) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mucin 4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Mucin 4 Antibody (YA3264) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mucin 4.

Host

Rabbit

Molecular Weight

Predicted band size: 542 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q99102

Gene ID

4585 [NCBI]

Immunogen

Recombinant protein of human MUC4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978). These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978). Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation

Subcellular Localization：Cell membrane; Single-pass membrane protein; Cell membrane; Secreted; Cell membrane; Single-pass membrane protein; Secreted; Secreted; Cell membrane; Single-pass membrane protein

Expression：
Tissue_specificity:This gene is expressed in the thymus, thyroid gland, lungs, trachea, esophagus, stomach, small intestine, colon, testes, prostate, ovaries, uterus, placenta, as well as mammary glands and salivary glands. It is also expressed in carcinomas originating from some of these epithelial tissues, such as lung cancer, squamous cell carcinoma of the upper respiratory and digestive tract, breast cancer, biliary tract cancer, colon cancer, and cervical cancer. It is expressed at very low levels or not at all in normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.

Isoforms & Post-Translational Modification：Q99102 has 14 isomers: Q99102-1: 542307 Da (predicted); Q99102-2: 429631 Da (predicted); Q99102-3: 504936 Da (predicted); Q99102-5: 436140 Da (predicted); Q99102-7: 434209 Da (predicted); Q99102-8: 433835 Da (predicted); Q99102-9: 424339 Da (predicted); Q99102-10: 536223 Da (predicted); Q99102-11: 432689 Da (predicted); Q99102-12: 125130 Da (predicted); Q99102-13: 130415 Da (predicted); Q99102-15: 431438 Da (predicted); Q99102-16: 455635 Da (predicted); Q99102-17: 517394 Da (predicted).
Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain;Highly O-glycosylated;Is predominantly N-glycosylated

Subunit：A heterodimeric complex, composed of a mucin-4 alpha chain and a cysteine-rich transmembrane mucin-4 beta chain. Mucin-4 beta chain interacts with ERBB2 via the EGF-like domain 1. In nonpolarized cells, associates with ERBB2 and ERBB3

Research Field

Microbiology

Synonyms

Mucin-4; Ascites sialoglycoprotein; ASGP; Pancreatic adenocarcinoma mucin; Testis mucin; Tracheobronchial mucin

Documentation

User Guide for Antibodies (1077 KB)