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ADX/FDX1 Antibody (YA7180)

(Synonyms: Adrenodoxin, ADX, Ferredoxin-1,LOH11CR1D,FDX, FDX1)
Customer Review

Based on 1 Customer Validation

ADX/FDX1 Antibody (YA7180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ADX/FDX1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • ADX/FDX1 Antibody (YA7180) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:200-1:1000 1:50

Product Details

Description

ADX/FDX1 Antibody (YA7180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ADX/FDX1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 13 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 19 kDa,
Species Reactivity Database
Immunogen

Synthetic peptide within Human ADX aa 51-100 / 184.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
    Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , C2C12(lane 3(20ug) and HepG2(lane 4(20ug) , HEK293(lane 5(20ug) using ADX/FDX1 Antibody (HY-P87493) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
    Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
    Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ADX/FDX1 is essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons[1][2].

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Tissue_Specificity: Highest levels in the adrenal gland (at protein level). Also detected in kidney and testis (at protein level)

  • Isoforms & Post-Translational Modification

    P10109: 184 amino acids, molecular weight 19393 Da.

  • Subunit

    Interacts with CYP11A1

  • SwissProt ID

    P10109

  • Synonyms

    Adrenodoxin, ADX, Ferredoxin-1,LOH11CR1D,FDX, FDX1

ADX/FDX1 Antibody (YA7180) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , C2C12(lane 3(20ug) and HepG2(lane 4(20ug) , HEK293(lane 5(20ug) using ADX/FDX1 Antibody (HY-P87493) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ADX/FDX1 Antibody (YA7180) Product Image
    1/16
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • ADX/FDX1 Antibody (YA7180) Product Image
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , C2C12(lane 3(20ug) and HepG2(lane 4(20ug) , HEK293(lane 5(20ug) using ADX/FDX1 Antibody (HY-P87493) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • Experimental Validation Results for ADX/FDX1 Antibody (YA7180)
  • ADX/FDX1 Antibody (YA7180) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SK-BR-3(lane 2(20ug) , C2C12(lane 3(20ug) and HepG2(lane 4(20ug) , HEK293(lane 5(20ug) using ADX/FDX1 Antibody (HY-P87493) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ADX/FDX1 Antibody (YA7180) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ADX/FDX1 Antibody (YA7180) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling ADX/FDX1 Antibody (HY-P87493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with ADX/FDX1 Antibody (HY-P87493) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ADX/FDX1 Antibody (YA7180) Product Image WB
  • ADX/FDX1 Antibody (YA7180) Product Image ICC
  • ADX/FDX1 Antibody (YA7180) Product Image ICC