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Cytokeratin 15 Antibody (YA3847)

(Synonyms: K15; CK15; K1CO; KRT1)

Cytokeratin 15 Antibody (YA3847) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Cytokeratin 15.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
  • Cytokeratin 15 Antibody (YA3847) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Cytokeratin 15 Antibody (YA3847) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Cytokeratin 15.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Immunogen

Purified recombinant fragment of KRT15 aa 272-456.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
    Immunocytochemistry analysis of HepG2 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
    Immunocytochemistry analysis of PANC-1 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Cytokeratin 15 (CK15), also known as keratin-15 (KRT15), has been described as a stem cell marker based on its expression in human hair follicles and other organs. In animal models, CK15 has been shown to be a marker expressed in self-renewing, regenerative progenitor stem cells. In the breast, CK15 expression was reported in resting undifferentiated ductal luminal cells, with evidence linking CK15 expression with immature and basal-like properties. As for human cancers, CK15 expression is associated with aggressive tumour features and poor clinical outcomes in endometrial and oesophageal cancers[1].

  • Expression


    Tissue specificity:
    Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the pal

  • Isoforms & Post-Translational Modification

    Cytokeratin 15 has 2 isomers: P19012-1: 49,212 Da (predicted); P19012-2: 28,602 Da (predicted).

  • Subunit

    Heterotetramer of two type I and two type II keratins (By similarity). Forms a heterodimer with KRT14. Interacts with PLEC isoform 1C, when in a heterodimer with KRT14. Interacts with NOD2

  • SwissProt ID

    P19012

  • Gene ID
    3866 [NCBI]
  • Synonyms

    K15; CK15; K1CO; KRT1

References

[1]. Lee DHY, et al. Cytokeratin 15 is a novel and independent predictor of poor outcome in luminal B HER2-negative breast carcinomas. Pathology. 2024 Oct;56(6):834-841.

Cytokeratin 15 Antibody (YA3847) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cytokeratin 15 Antibody (YA3847) Product Image
    1/16
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
  • Cytokeratin 15 Antibody (YA3847) Product Image
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
  • Experimental Validation Results for Cytokeratin 15 Antibody (YA3847)
  • Cytokeratin 15 Antibody (YA3847) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cytokeratin 15 Antibody (YA3847) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of PANC-1 cells labeling KRT15 with KRT15 Antibody (HY-P84150) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with KRT15 Antibody (HY-P84150)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cytokeratin 15 Antibody (YA3847) Product Image ICC
  • Cytokeratin 15 Antibody (YA3847) Product Image ICC