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Methylmalonyl Coenzyme A mutase Antibody (YA295)

(Synonyms: MUT, MMUT, MCM, Methylmalonyl-CoA isomerase)
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Based on 1 Customer Validation

Methylmalonyl Coenzyme A mutase Antibody (YA295) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Methylmalonyl Coenzyme A mutase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Methylmalonyl Coenzyme A mutase Antibody (YA295)
  • Methylmalonyl Coenzyme A mutase Antibody (YA295) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:200-1:500

Product Details

Description

Methylmalonyl Coenzyme A mutase Antibody (YA295) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Methylmalonyl Coenzyme A mutase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 83 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Methylmalonyl Coenzyme A mutase.AA range:45-95.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Methylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Methylmalonyl Coenzyme A mutase Antibody (YA295)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Methylmalonyl Coenzyme A mutase Antibody (HY-P80222) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Methylmalonyl Coenzyme A mutase catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle

  • Subcellular Localization

    Mitochondrion matrix; Mitochondrion; Cytoplasm

  • Subunit

    Homodimer (PubMed:20876572). Interacts (the apoenzyme form) with MMAA; the interaction is GTP dependent (PubMed:20876572, PubMed:21138732, PubMed:28943303)

  • SwissProt ID

    P22033

  • Gene ID
    4594 [NCBI]
  • Synonyms

    MUT, MMUT, MCM, Methylmalonyl-CoA isomerase

  • Research Field

    Signal Transduction

Methylmalonyl Coenzyme A mutase Antibody (YA295) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Methylmalonyl Coenzyme A mutase Antibody (HY-P80222) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Methylmalonyl Coenzyme A mutase Antibody (YA295) Product Image
    1/16
  • Experimental Validation Results for Methylmalonyl Coenzyme A mutase Antibody (YA295)
  • Methylmalonyl Coenzyme A mutase Antibody (YA295) Product Image
  • Experimental Validation Results for Methylmalonyl Coenzyme A mutase Antibody (YA295)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Methylmalonyl Coenzyme A mutase Antibody (HY-P80222) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Methylmalonyl Coenzyme A mutase Antibody (YA295)
  • Methylmalonyl Coenzyme A mutase Antibody (YA295) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Methylmalonyl Coenzyme A mutase Antibody (HY-P80222) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Methylmalonyl Coenzyme A mutase Antibody (YA295) Product Image WB