PTBP1 Antibody (YA3189)(PBS only)

Art. -Nr.: HY-P83444A: Data Sheet User Guide for Antibodies
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PTBP1 Antibody (YA3189) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PTBP1.

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100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

PTBP1 Antibody (YA3189) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PTBP1.

Host

Rabbit

Molekulargewicht

Predicted band size: 57 kDa;

Observed band size: 57 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P26599

Gene ID

5725 [NCBI]

Immunogen

A synthetic peptide of human PTBP1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	200-1:500
FC FC: Flow Cytometry	200-1:2000

Reinheit	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9 (PubMed:14966131). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806)

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：P26599 has 3 isomers: P26599-3: 59633 Da (predicted); P26599-1: 57221 Da (predicted); P26599-2: 59037 Da (predicted).

Subunit：Monomer. Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1. Interacts with RAVER1 and SFPQ. Interacts with IVNS1ABP (via BACK domain); the interaction is direct (PubMed:30538201)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/cho-cell.html [Content Brief]