AMSH Antibody (YA2707)(PBS only)

Art. -Nr.: HY-P82962A: Data Sheet User Guide for Antibodies
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AMSH Antibody (YA2707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AMSH.

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

AMSH Antibody (YA2707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AMSH.

Host

Rabbit

Molekulargewicht

Predicted band size: 48 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95630

Gene ID

10617 [NCBI]

Immunogen

A synthetic peptide of human AMSH

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Reinheit	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:15314065, PubMed:23542699, PubMed:34425109). Does not cleave 'Lys-48'-linked polyubiquitin chains (PubMed:15314065). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF (PubMed:10383417). Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7 (PubMed:11483516). Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes (PubMed:15314065, PubMed:17261583). Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization (PubMed:15314065, PubMed:17261583). Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways (PubMed:23542699)

Subcellular Localization：Nucleus; Membrane; Peripheral membrane protein; Cytoplasm; Early endosome

Expression：
Tissue_specificity:Ubiquitously expressed

Isoforms & Post-Translational Modification：O95630 has 2 isomers: O95630-1: 48077 Da (predicted); O95630-2: 39596 Da (predicted).
Phosphorylated after BMP type I receptor activation;Ubiquitinated by SMURF2 in the presence of RNF11

Subunit：Interacts with STAM (PubMed:10383417, PubMed:15314065). Interacts with SMAD6 and SMAD7 (PubMed:11483516). Interacts with CHMP3; the interaction appears to relieve the autoinhibition of CHMP3 (PubMed:17146056, PubMed:17261583).

Research Field

Cell Biology

Synonyms

AMSH; MICCAP

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/vero-cell.html [Content Brief]