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Cat. No.: HY-P82962A

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AMSH Antibody (YA2707)(PBS only)

Name: AMSH Antibody (YA2707)(PBS only)
Brand: MedChemExpress
SKU: HY-P82962A
Availability: PreOrder

(Synonyms: AMSH; MICCAP)

AMSH Antibody (YA2707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AMSH.

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot
Dilution Ratio	1:500-1:1000

Description

AMSH Antibody (YA2707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AMSH.

Host Rabbit
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 48 kDa

Immunogen

A synthetic peptide of human AMSH

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Western blot analysis of extracts from A549(lane 2(20ug) ，HL-60(lane 3(20ug) and HepG2(lane 4(20ug) using AMSH Antibody (HY-P82962) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Function

AMSH is a Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization. Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways^[1]^[2]^[3]^[4]^[5]^[6].
Subcellular Localization

Nucleus; Membrane; Peripheral membrane protein; Cytoplasm; Early endosome
Expression

Tissue_specificity:Ubiquitously expressed
Isoforms & Post-Translational Modification

O95630 has 2 isomers: O95630-1: 48077 Da (predicted); O95630-2: 39596 Da (predicted).
Phosphorylated after BMP type I receptor activation;Ubiquitinated by SMURF2 in the presence of RNF11
Subunit

Interacts with STAM (PubMed:10383417, PubMed:15314065). Interacts with SMAD6 and SMAD7 (PubMed:11483516). Interacts with CHMP3; the interaction appears to relieve the autoinhibition of CHMP3 (PubMed:17146056, PubMed:17261583).
SwissProt ID

O95630
Gene ID

10617 [NCBI]
Synonyms

AMSH; MICCAP
Research Field

Cell Biology