XPA Antibody
(Synonyms: XPAC, XPA, DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein)XPA Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to XPA.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
XPA Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to XPA.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 31 kDa
Synthetic peptide corresponding to the C-term region of human XPA.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
XPA is involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3. Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH[1][2].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Expressed in various cell lines and in skin fibroblasts. -
Isoforms & Post-Translational Modification
XPA has an amino acid length of 273, molecular weight is 31368 Da.
ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage. -
Subunit
Interacts with GPN1.
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SwissProt ID
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Synonyms
XPAC, XPA, DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein
Documentation
References
[1]. Pan YR, et al. UV-dependent interaction between Cep164 and XPA mediates localization of Cep164 at sites of DNA damage and UV sensitivity. Cell Cycle. 2009 Feb 15;8(4):655-64. [Content Brief]
[2]. Kokic G, et al. Structural basis of TFIIH activation for nucleotide excision repair. Nat Commun. 2019 Jun 28;10(1):2885. [Content Brief]
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