Smad1 Antibody (YA7554)
(Synonyms: BSP1, MADH1, MADR1, SMAD1, Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1, SMAD 1, Smad1, hSMAD1, BSP-1)Smad1 Antibody (YA7554) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Smad1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
Smad1 Antibody (YA7554) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Smad1.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 52 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 52 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Smad1 is a BMP-responsive receptor-regulated Smad whose phosphorylation is rapidly induced by BMP2, driving nuclear accumulation and BMP signal transduction[1]. Mechanistically, BMP receptors phosphorylate Smad1, Smad1 associates with Smad4, and the complex enters the nucleus to activate transcription[2]. In BMP-responsive promoters such as ID1, Smad1/Smad4-dependent DNA binding supports BMP-specific transcriptional activation[3]. In developmental models, canonical Smad1/5 signaling is required for endochondral bone formation, and SMAD1/5 activity controls spinal neural progenitor division modes[4][5]. In disease-relevant models, BMP-Smad1 signaling participates in DNA damage response and oncogenesis through the Atm-p53 pathway[6]. Compared with related BMP-regulated isoforms, Smad9 shows lower transcriptional activity than Smad1 or Smad5 despite Smad4 association and target-DNA binding, distinguishing Smad1 as a stronger BMP transcriptional effector[7]. For experimental applications, Smad6 blocks BMP/Smad1 signaling by competing with Smad4, while dorsomorphin and LDN-193189 inhibit BMP-mediated Smad signaling in C2C12 cells[2][8].
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Subcellular Localization
Cytoplasm,Nucleus
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Expression
Tissue_Specificity: Ubiquitous. Highest expression seen in the heart and skeletal muscle -
Isoforms & Post-Translational Modification
Q15797 has two isomers: Q15797-1: 52260 Da (predicted); Q15797-2: 15406 Da (predicted).
Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4丨Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation -
Subunit
Found in a complex with SMAD4 and YY1
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SwissProt ID
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Synonyms
BSP1, MADH1, MADR1, SMAD1, Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1, SMAD 1, Smad1, hSMAD1, BSP-1
Documentation
References
[1]. Hoodless PA, et al. MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell. 1996 May 17;85(4):489-500. [Content Brief]
[2]. Hata A, et al. Smad6 inhibits BMP/Smad1 signaling by specifically competing with the Smad4 tumor suppressor. Genes Dev. 1998 Jan 15;12(2):186-97. [Content Brief]
[3]. López-Rovira T, et al. Direct binding of Smad1 and Smad4 to two distinct motifs mediates bone morphogenetic protein-specific transcriptional activation of Id1 gene. J Biol Chem. 2002 Feb 1;277(5):3176-85. [Content Brief]
[4]. Retting KN, et al. BMP canonical Smad signaling through Smad1 and Smad5 is required for endochondral bone formation. Development. 2009 Apr;136(7):1093-104. [Content Brief]
[5]. Le Dréau G, et al. The strength of SMAD1/5 activity determines the mode of stem cell division in the developing spinal cord. J Cell Biol. 2014 Feb 17;204(4):591-605. [Content Brief]
[6]. Chau JF, et al. A crucial role for bone morphogenetic protein-Smad1 signalling in the DNA damage response. Nat Commun. 2012 May 15;3:836. [Content Brief]
[7]. Tsukamoto S, et al. Smad9 is a new type of transcriptional regulator in bone morphogenetic protein signaling. Sci Rep. 2014 Dec 23;4:7596. [Content Brief]
[8]. Li B, et al. Differences in endoplasmic reticulum stress signalling kinetics determine cell survival outcome through activation of MKP-1. Cell Signal. 2011 Jan;23(1):35-45. [Content Brief]
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