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FOXI1 Antibody (YA7787)(PBS only)

(Synonyms: FKH10; FKHL10; FREAC-6; FREAC6; HFH-3; HFH3)

FOXI1 Antibody (YA7787) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXI1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
  • FOXI1 Antibody (YA7787)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000 1:150 1:100 1:100

Product Details

Description

FOXI1 Antibody (YA7787) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXI1.

  • Host Mouse
  • Species Reactivity
    Human, Mouse
  • Calculated Molecular Weight Predicted band size: 40.8 kDa
Immunogen

Human recombinant protein fragment corresponding to amino acids 111-350 of human FOXI1 produced in E.coli.

Purification

Affinity purified

Conjugation

Non-conjugated

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
    Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
    Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    FOXI1 transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_Specificity: Expressed in kidney

  • Isoforms & Post-Translational Modification

    Q12951 has two isomers: Q12951-1: 40973 Da (predicted); Q12951-2: 30814 Da (predicted).

  • SwissProt ID

    Q12951

  • Synonyms

    FKH10; FKHL10; FREAC-6; FREAC6; HFH-3; HFH3

FOXI1 Antibody (YA7787)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FOXI1 Antibody (YA7787)(PBS only) Product Image
    1/16
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
  • FOXI1 Antibody (YA7787)(PBS only) Product Image
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
  • Experimental Validation Results for FOXI1 Antibody (YA7787)(PBS only)
  • FOXI1 Antibody (YA7787)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FOXI1 Antibody (YA7787)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling FOXI1 Antibody (HY-P88103) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD47 Antibody (HY-P88103) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FOXI1 Antibody (YA7787)(PBS only) Product Image ICC
  • FOXI1 Antibody (YA7787)(PBS only) Product Image ICC