ISCU Antibody (YA8012)
(Synonyms: 2310020H20Rik; HML; hnifU; ISU2; NIFU; NIFUN)
Based on 1 Customer Validation
ISCU Antibody (YA8012) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ISCU.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:2000 | 1:150-500 |
Product Details
ISCU Antibody (YA8012) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ISCU.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 15.1 kDa
Full length human recombinant protein of human ISCU produced in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ISCU is a Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (Probable). Exists as two slow interchanging conformational states, a structured (S) and disordered (D) form. May modulate NFS1 desulfurase activity in a zinc-dependent manner. Modulates the interaction between FXN and the cysteine desulfurase complex |Cytoplasmic scaffold protein, of the cytoplasmic core iron-sulfur cluster (ISC) assembly complex that provides the structural architecture on which the Fe-S clusters are assembled and may be involved in the cytoplasmic iron-sulfur protein biogenesis[1][2][3][4][5].
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Subcellular Localization
Mitochondrion,Cytoplasm,Nucleus
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Expression
Tissue_Specificity: Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta -
Isoforms & Post-Translational Modification
Q9H1K1 has two isomers: Q9H1K1-1: 17999 Da (predicted); Q9H1K1-2: 15263 Da (predicted).
Phosphorylation at Ser-14 is required for ISCU protein stabilization in the cytosol, whereas dephosphorylation of Ser-14, due to the inhibition of mTORC1 (mammalian target of rapamycin complex 1) complex, leads to degradation of the precursor form and ultimately to a decrease in the mitochondrial mature form丨Cysteine persulfide is reduced by thiol-containing molecules such as glutathione and L-cysteine -
Subunit
Homodimer; Tyr-35-mediated dimerization of two iron- and sulfide-containing ISCU subunit bind to the cysteine desulfurase complex (PubMed:31101807, PubMed:34824239)
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SwissProt ID
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Synonyms
2310020H20Rik; HML; hnifU; ISU2; NIFU; NIFUN
Documentation
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