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EEF2K Antibody (YA6551)

(Synonyms: C86191 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody; Calmodulin dependent protein kinase III antibody; cb365 antibody; EC 2.7.11.20 antibody; eEF 2 kinase antibody; eEF 2K antibody; eEF-2 kinase antibody; C86191 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody; Calmodulin dependent protein kinase III antibody; cb365 antibody; EC 2.7.11.20 antibody; eEF 2 kinase antibody; eEF 2K antibody; eEF-2 kinase antibody; eEF-2K antibody; Eef2k antibody; EEF2K protein antibody; EF2K_HUMAN antibody; Elongation factor 2 kinase antibody; Eukaryotic elongation factor 2 kinase antibody; fa04b08 antibody; HSU93850 antibody; kinase eEF2K antibody; MGC45041 antibody; SMEF2K antibody; wu:fa04b08 antibody; zgc:100973 antibody; )
Customer Review

Based on 1 Customer Validation

EEF2K Antibody (YA6551) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EEF2K.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EEF2K Antibody (YA6551)
  • EEF2K Antibody (YA6551) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:5000-1:10000 1:100 1:1000

Product Details

Description

EEF2K Antibody (YA6551) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EEF2K.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 82-90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 82 kDa
Immunogen

Recombinant protein within human EEF2K aa 476-725.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EEF2K Antibody (YA6551)
    Western blot analysis of extracts from K562 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using EEF2K Antibody (HY-P86858) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    EEF2K is a Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced

  • Isoforms & Post-Translational Modification

    O00418: 725 amino acids, molecular weight 82144 Da.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex

  • Subunit

    Monomer or homodimer (Probable)

  • SwissProt ID

    O00418

  • Gene ID
    29904 [NCBI]
  • Synonyms

    C86191 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody; Calmodulin dependent protein kinase III antibody; cb365 antibody; EC 2.7.11.20 antibody; eEF 2 kinase antibody; eEF 2K antibody; eEF-2 kinase antibody; C86191 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody; Calmodulin dependent protein kinase III antibody; cb365 antibody; EC 2.7.11.20 antibody; eEF 2 kinase antibody; eEF 2K antibody; eEF-2 kinase antibody; eEF-2K antibody; Eef2k antibody; EEF2K protein antibody; EF2K_HUMAN antibody; Elongation factor 2 kinase antibody; Eukaryotic elongation factor 2 kinase antibody; fa04b08 antibody; HSU93850 antibody; kinase eEF2K antibody; MGC45041 antibody; SMEF2K antibody; wu:fa04b08 antibody; zgc:100973 antibody;

EEF2K Antibody (YA6551) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using EEF2K Antibody (HY-P86858) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • EEF2K Antibody (YA6551) Product Image
    1/16
  • Experimental Validation Results for EEF2K Antibody (YA6551)
  • EEF2K Antibody (YA6551) Product Image
  • Experimental Validation Results for EEF2K Antibody (YA6551)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using EEF2K Antibody (HY-P86858) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EEF2K Antibody (YA6551)
  • EEF2K Antibody (YA6551) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using EEF2K Antibody (HY-P86858) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • EEF2K Antibody (YA6551) Product Image WB