Histone H4 (acetyl K8) Antibody (YA365)

製品番号: HY-P80180: データシート SDS COA User Guide for Antibodies
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Histone H4 (acetyl K8) Antibody (YA365) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H4 (acetyl K8).

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容量	価格（税別）	在庫状況	数量
10 μL	$94	在庫あり	Estimated Time of Arrival: December 31
50 μL	$249	在庫あり	Estimated Time of Arrival: December 31
100 μL	$405	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

•Adv Sci (Weinh). 2026 Apr;13(23):e18185.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Histone H4 (acetyl K8) Antibody (YA365) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H4 (acetyl K8).

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 11 kDa;

Observed band size: 14/16 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P62805

Gene ID

121504 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Histone H4.AA range:1-20.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:5000
ChIP ChIP: Chromatin Immunoprecipitation	Use 0.5~2 μg for 25 μg of chromatin

Sensitivity	Endogenous	純度	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Acetylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Histone H4 Antibody (HY-P80180) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Subcellular Localization：Nucleus; Chromosome

Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

RRID

AB_3102849

Database

Entrez Gene: 121504 Human ; 100041230 Mouse ; 291152 Rat

SwissProt: P62805 Human ; P62806 Mouse ; P62804 Rat

OMIM: 619758 Human

Research Field

Epigenetics and Nuclear Signaling

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)