Transcription Initiation Factor IIB Antibody (YA5166)
(Synonyms: GTF2B; TF2B; TFIIB; Transcription initiation factor IIB; General transcription factor TFIIB; S300-II)Transcription Initiation Factor IIB Antibody (YA5166) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Transcription Initiation Factor IIB.
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Host:
Mouse
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:500-1:1000 | 1:100-1:500 |
Product Details
Transcription Initiation Factor IIB Antibody (YA5166) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Transcription Initiation Factor IIB.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat, Bovine, Chicken, Dog, Pig
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Calculated Molecular Weight Predicted band size: 35 kDa;
Purified recombinant human TFIIB protein fragments expressed in E.coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Transcription Initiation Factor IIB is a General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Tissue_specificity:It is expressed in the inner cell mass that forms the blastocyst (PubMed:24441171) . It was not detected (at the protein level) in the outer thin trophoblast cells (PubMed:24441171) . -
Subunit
Found in a ternary complex with TATA box-bound TBP (PubMed:10619841, PubMed:29158257, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
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SwissProt ID
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Synonyms
GTF2B; TF2B; TFIIB; Transcription initiation factor IIB; General transcription factor TFIIB; S300-II
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