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Superoxide Dismutase 3 Antibody (YA2420)

(Synonyms: EC SOD; Extracellular superoxide dismutase [Cu Zn]; Extracellular superoxide dismutase precursor; SOD3; Superoxide dismutase 3 extracellular)
Customer Review

Based on 1 Customer Validation

Superoxide Dismutase 3 Antibody (YA2420) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Superoxide Dismutase 3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

Superoxide Dismutase 3 Antibody (YA2420) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Superoxide Dismutase 3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Species Reactivity Database

Entrez Gene: 6649 Human

SwissProt: P08294 Human

Immunogen

A synthesized peptide derived from human Superoxide Dismutase 3 aa200-240.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    Superoxide Dismutase 3 is a Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen

  • Subcellular Localization

    Secreted, extracellular space; Golgi apparatus, trans-Golgi network

  • Expression


    Tissue_specificity:Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid

  • Subunit

    Homotetramer (PubMed:19289127). Directly interacts with ATP7A; this interaction is copper-dependent and is required for SOD3 activity (PubMed:16371425)

  • SwissProt ID

    P08294

  • Gene ID
    6649 [NCBI]
  • Synonyms

    EC SOD; Extracellular superoxide dismutase [Cu Zn]; Extracellular superoxide dismutase precursor; SOD3; Superoxide dismutase 3 extracellular

  • Research Field

    Cell Biology

Superoxide Dismutase 3 Antibody (YA2420) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    1/16
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Experimental Validation Results for Superoxide Dismutase 3 Antibody (YA2420)
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pleomorphic adenoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreatic cancer tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Superoxide Dismutase antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82675, 1:500 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Superoxide Dismutase 3 Antibody (YA2420) Product Image IHC-P
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image IHC-P
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image IHC-P
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image IHC-P
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image IHC-P
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image IHC-P
  • Superoxide Dismutase 3 Antibody (YA2420) Product Image IHC-P