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MGMT Antibody (YA293)

(Synonyms: Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, MGMT)
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Based on 1 Customer Validation

MGMT Antibody (YA293) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MGMT.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MGMT Antibody (YA293)
  • MGMT Antibody (YA293) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000 1:50-1:200

Product Details

Description

MGMT Antibody (YA293) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MGMT.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Species Reactivity Database

Entrez Gene: 4255 Human

SwissProt: P16455 Human

Immunogen

Synthetic peptide corresponding to Human MGMT.AA range:1-140.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102864

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MGMT Antibody (YA293)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and HEK293(lane 4(20μg) using MGMT (HY-P80224) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    MGMT encodes O6-methylguanine-DNA methyltransferase, also called AGT, a DNA repair protein that removes alkyl groups from O6-alkylguanine adducts[1]. Mechanistically, MGMT repairs cytotoxic O6-methylguanine and O6-chloroethylguanine lesions induced by methylating or chloroethylating agents, thereby limiting DNA damage signaling and alkylating-drug cytotoxicity[2][3]. In glioma, MGMT promoter methylation silences MGMT expression and predicts clinical response to alkylating agents, including temozolomide[4][5]. Compared with separate isoform targets, the retrieved literature describes MGMT/AGT as the same repair protein, so isoform-specific claims require additional evidence[1]. For experimental applications, O6-benzylguanine and O6- (4-bromothenyl) guanine have been used to inactivate MGMT, but normal-tissue MGMT inhibition can increase alkylating-drug toxicity[6].

  • Subcellular Localization

    Nucleus

  • SwissProt ID

    P16455

  • Gene ID
    4255 [NCBI]
  • Synonyms

    Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, MGMT

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Fang Q. The Versatile Attributes of MGMT: Its Repair Mechanism, et al. The Versatile Attributes of MGMT: Its Repair Mechanism, Crosstalk with Other DNA Repair Pathways, and Its Role in Cancer. Cancers (Basel). 2024 Jan 11;16(2):331. [Content Brief]

[2]. Kaina B, et al. MGMT: key node in the battle against genotoxicity, carcinogenicity and apoptosis induced by alkylating agents. DNA Repair (Amst). 2007 Aug 1;6(8):1079-99. [Content Brief]

[3]. Fan CH, et al. O6-methylguanine DNA methyltransferase as a promising target for the treatment of temozolomide-resistant gliomas. Cell Death Dis. 2013 Oct 24;4(10):e876. [Content Brief]

[4]. Esteller M, et al. Inactivation of the DNA-repair gene MGMT and the clinical response of gliomas to alkylating agents. N Engl J Med. 2000 Nov 9;343(19):1350-4. [Content Brief]

[5]. Hegi ME, et al. MGMT gene silencing and benefit from temozolomide in glioblastoma. N Engl J Med. 2005 Mar 10;352(10):997-1003. [Content Brief]

[6]. Kaina B, et al. Targeting O⁶-methylguanine-DNA methyltransferase with specific inhibitors as a strategy in cancer therapy. Cell Mol Life Sci. 2010 Nov;67(21):3663-81. [Content Brief]

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MGMT Antibody (YA293) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and HEK293(lane 4(20μg) using MGMT (HY-P80224) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MGMT Antibody (YA293) Product Image
    1/16
  • Experimental Validation Results for MGMT Antibody (YA293)
  • MGMT Antibody (YA293) Product Image
  • Experimental Validation Results for MGMT Antibody (YA293)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and HEK293(lane 4(20μg) using MGMT (HY-P80224) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MGMT Antibody (YA293)
  • MGMT Antibody (YA293) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and HEK293(lane 4(20μg) using MGMT (HY-P80224) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MGMT Antibody (YA293) Product Image WB