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SLFN11 Antibody

(Synonyms: SLFN8/9; SLFN11)
Customer Review

Based on 1 Customer Validation

SLFN11 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLFN11.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    1.Supplied in PBS, 0.05% proclin300, 50% glycerol,pH7.3.
    2.Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SLFN11 Antibody
  • SLFN11 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

SLFN11 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLFN11.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 103 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 103 kDa
Immunogen

Recombinant fusion protein

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718755

Product Properties

  • Appearance

    Liquid

  • Formulation

    1.Supplied in PBS, 0.05% proclin300, 50% glycerol,pH7.3.
    2.Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SLFN11 Antibody
    Western blot analysis of extracts from Jurkat (lane 2(20μg), MCF-7 (lane 3(20μg), THP-1 (lane 4(20μg), K562 (lane 5(20μg) using SLFN11 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    SLFN11 is an Inhibitor of DNA replication that promotes cell death in response to DNA damage. Acts as a guardian of the genome by killing cells with defective replication. Persistently blocks stressed replication forks by opening chromatin across replication initiation sites at stressed replication forks, possibly leading to unwind DNA ahead of the MCM helicase and block fork progression, ultimately leading to cell death. Upon DNA damage, inhibits translation of ATR or ATM based on distinct codon usage without disrupting early DNA damage response signaling. Antiviral restriction factor with manganese-dependent type II tRNA endoribonuclease. A single tRNA molecule is bound and cleaved by the SLFN11 dimer. Specifically abrogates the production of retroviruses such as human immunodeficiency virus 1 (HIV-1) by acting as a specific inhibitor of the synthesis of retroviruses encoded proteins in a codon-usage-dependent manner. Impairs the replication of human cytomegalovirus (HCMV) and some Flaviviruses. Exploits the unique viral codon bias towards A/T nucleotides. Also acts as an interferon (IFN)-induced antiviral protein which acts as an inhibitor of retrovirus protein synthesis[1][2][3][4][5][6][7].

  • Subcellular Localization

    Nucleus; Chromosome

  • Expression


    Tissue_specificity:Exhibits a wider expression range in ovarian and colon adenocarcinoma than in their corresponding healthy tissues

    Induction:Down-regulated in small cell lung cancer (SCLC) cells resistant to PARP inhibitor drugs (PubMed:26625211, PubMed:27440269, PubMed:28196596, PubMed:28212573) . Up-regulated by type I interferons, poly-IC and poly-dAdT (PubMed:20956525, PubMed:23000900)

  • Subunit

    Homodimer (PubMed:36115853). Interacts with MCM3 (PubMed:29395061). Interacts with DHX9 (PubMed:26625211, PubMed:29395061). Interacts with RPA1 (PubMed:26658330, PubMed:29395061)

  • SwissProt ID

    Q7Z7L1

  • Gene ID
    91607 [NCBI]
  • Synonyms

    SLFN8/9; SLFN11

SLFN11 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), MCF-7 (lane 3(20μg), THP-1 (lane 4(20μg), K562 (lane 5(20μg) using SLFN11 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SLFN11 Antibody Product Image
    1/16
  • Experimental Validation Results for SLFN11 Antibody
  • SLFN11 Antibody Product Image
  • Experimental Validation Results for SLFN11 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), MCF-7 (lane 3(20μg), THP-1 (lane 4(20μg), K562 (lane 5(20μg) using SLFN11 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLFN11 Antibody
  • SLFN11 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), MCF-7 (lane 3(20μg), THP-1 (lane 4(20μg), K562 (lane 5(20μg) using SLFN11 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SLFN11 Antibody Product Image WB