SLFN11 Antibody

Cat. No.: HY-P83769: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

SLFN11 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLFN11.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

SLFN11 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLFN11.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 103 kDa;

Observed band size: 103 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q7Z7L1

Gene ID

91607 [NCBI]

Immunogen

Recombinant fusion protein

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

1.Supplied in PBS, 0.05% proclin300, 50% glycerol,pH7.3.
2.Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Please refer to the lot-specific COA for specific buffer information.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat (lane 2(20μg), MCF-7 (lane 3(20μg), THP-1 (lane 4(20μg), K562 (lane 5(20μg) using SLFN11 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Inhibitor of DNA replication that promotes cell death in response to DNA damage (PubMed:22927417, PubMed:26658330, PubMed:29395061). Acts as a guardian of the genome by killing cells with defective replication (PubMed:29395061). Persistently blocks stressed replication forks by opening chromatin across replication initiation sites at stressed replication forks, possibly leading to unwind DNA ahead of the MCM helicase and block fork progression, ultimately leading to cell death (PubMed:29395061). Upon DNA damage, inhibits translation of ATR or ATM based on distinct codon usage without disrupting early DNA damage response signaling (PubMed:30374083). Antiviral restriction factor with manganese-dependent type II tRNA endoribonuclease (PubMed:36115853). A single tRNA molecule is bound and cleaved by the SLFN11 dimer (PubMed:36115853). Specifically abrogates the production of retroviruses such as human immunodeficiency virus 1 (HIV-1) by acting as a specific inhibitor of the synthesis of retroviruses encoded proteins in a codon-usage-dependent manner (PubMed:23000900). Impairs the replication of human cytomegalovirus (HCMV) and some Flaviviruses (PubMed:35105802, PubMed:36115853). Exploits the unique viral codon bias towards A/T nucleotides (PubMed:23000900). Also acts as an interferon (IFN)-induced antiviral protein which acts as an inhibitor of retrovirus protein synthesis (PubMed:23000900)

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:Exhibits a wider expression range in ovarian and colon adenocarcinoma than in their corresponding healthy tissues

Induction:Down-regulated in small cell lung cancer (SCLC) cells resistant to PARP inhibitor drugs (PubMed:26625211, PubMed:27440269, PubMed:28196596, PubMed:28212573) . Up-regulated by type I interferons, poly-IC and poly-dAdT (PubMed:20956525, PubMed:23000900)

Subunit：Homodimer (PubMed:36115853). Interacts with MCM3 (PubMed:29395061). Interacts with DHX9 (PubMed:26625211, PubMed:29395061). Interacts with RPA1 (PubMed:26658330, PubMed:29395061)

RRID

AB_3718755

Synonyms

SLFN8/9; SLFN11

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mm96l-cell.html [Content Brief]

SLFN11 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SLFN11 Antibody

Product Detail

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Background

Documentation

References

SLFN11 Antibody Related Classifications

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