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RAC1/2/3 Antibody (YA3290)

(Synonyms: Cell migration-inducing gene 5 protein; EN-7; GX; p21-Rac1; p21-Rac2; p21-Rac3; RAC1; RAC2; RAC3; TC25)
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Based on 1 Customer Validation

RAC1/2/3 Antibody (YA3290) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAC1/2/3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
  • RAC1/2/3 Antibody (YA3290) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

RAC1/2/3 Antibody (YA3290) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAC1/2/3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Rac1/2/3 aa1-192.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105654

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Hela (lane 2, 15 μg), HUVEC (lane 3, 15 μg), C6 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using Rac Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using RAC1/2/3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    RAC1/2/3 is a P63000: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity.
    P15153: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In its active state, binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Regulatory subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2- )
    P60763: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner
    [1][2][3][4][5][6][7].

  • Subcellular Localization

    P63000: Cell membrane; Lipid-anchor; Cytoplasmic side; Melanosome; Cytoplasm; Cell projection, lamellipodium; Cell projection, dendrite; Synapse; Nucleus
    P15153: Cytoplasm
    P60763: Cytoplasm; Endomembrane system; Cell projection, lamellipodium; Cytoplasm, perinuclear region; Cell membrane; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity: P63000: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues
    P15153: Hematopoietic specific
    P60763: Highest levels in brain, also detected in heart, placenta and pancreas
    Induction: P60763: Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation

  • Isoforms & Post-Translational Modification

    P63000 has 2 isomers: P63000-1: 21450 Da (predicted); P63000-2: 23467 Da (predicted).
    GTP-bound active form is ubiquitinated at Lys-147 by HACE1, leading to its degradation by the proteasome;Phosphorylated by AKT at Ser-71;Ubiquitinated at Lys-166 in a FBXL19-mediated manner; leading to proteasomal degradation;(Microbial infection) AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo;(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly;(Microbial infection) Glucosylated at Thr-35 by C.difficile toxins TcdA and TcdB in the colonic epithelium, and by P.sordellii toxin TcsL in the vascular endothelium (PubMed:19744486, PubMed:24905543, PubMed:7775453, PubMed:7777059, PubMed:8626575). Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death, resulting in the loss of colonic epithelial barrier function (PubMed:7775453, PubMed:7777059);(Microbial infection) Glycosylated (O-GlcNAcylated) at Thr-35 by C.novyi toxin TcdA (PubMed:8810274). O-GlcNAcylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption (PubMed:8810274);(Microbial infection) Palmitoylated by the N-epsilon-fatty acyltransferase F2 chain of V.cholerae toxin RtxA (PubMed:29074776). Palmitoylation inhibits activation by guanine nucleotide exchange factors (GEFs), preventing Rho GTPase signaling (PubMed:29074776)

  • Subunit

    P63000: Interacts with NISCH. Interacts with PIP5K1A. Interacts with the GTP-bound form of RAB7A. Interacts with SRGAP2.
    P15153: Interacts with DOCK2, which may activate it. Interacts with S100A8 and calprotectin (S100A8/9) (PubMed:10559471, PubMed:15642721).
    P60763: Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it (PubMed:16982419). Interacts with C1D (PubMed:9852280). Interacts (via C-terminal region) with CIB1; the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion (PubMed:11756406). Interacts with NRBP (PubMed:11956649)

  • SwissProt ID

    P63000 / P15153 / P60763

  • Gene ID
    5881 [NCBI]
  • Synonyms

    Cell migration-inducing gene 5 protein; EN-7; GX; p21-Rac1; p21-Rac2; p21-Rac3; RAC1; RAC2; RAC3; TC25

  • Research Field

    Signal Transduction

RAC1/2/3 Antibody (YA3290) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Hela (lane 2, 15 μg), HUVEC (lane 3, 15 μg), C6 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using Rac Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using RAC1/2/3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RAC1/2/3 Antibody (YA3290) Product Image
    1/16
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
  • RAC1/2/3 Antibody (YA3290) Product Image
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Hela (lane 2, 15 μg), HUVEC (lane 3, 15 μg), C6 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using Rac Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using RAC1/2/3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
  • Experimental Validation Results for RAC1/2/3 Antibody (YA3290)
  • RAC1/2/3 Antibody (YA3290) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Hela (lane 2, 15 μg), HUVEC (lane 3, 15 μg), C6 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using Rac Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RAC1/2/3 Antibody (YA3290) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using RAC1/2/3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RAC1/2/3 Antibody (YA3290) Product Image WB
  • RAC1/2/3 Antibody (YA3290) Product Image WB