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von Willebrand Factor Antibody (YA6094)

Customer Review

Based on 1 Customer Validation

von Willebrand Factor Antibody (YA6094) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to von Willebrand Factor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • von Willebrand Factor Antibody (YA6094) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:4000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

von Willebrand Factor Antibody (YA6094) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to von Willebrand Factor.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 280 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 309 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719148

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg) and Rat spleen tissue (lane3, 20μg) using von Willebrand Factor Antibody (HY-P86402). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    von Willebrand Factor is important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma

  • Subcellular Localization

    Secreted; Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:plasma

  • Isoforms & Post-Translational Modification

    P04275 has 2 isomers: P04275-1: 309265 Da (predicted); P04275-2: 38745 Da (predicted).
    All cysteine residues are involved in intrachain or interchain disulfide bonds;N- and O-glycosylated

  • Subunit

    Multimeric. Interacts with F8

  • SwissProt ID

    P04275

  • Gene ID
    7450 [NCBI]

von Willebrand Factor Antibody (YA6094) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg) and Rat spleen tissue (lane3, 20μg) using von Willebrand Factor Antibody (HY-P86402). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • von Willebrand Factor Antibody (YA6094) Product Image
    1/16
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • von Willebrand Factor Antibody (YA6094) Product Image
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg) and Rat spleen tissue (lane3, 20μg) using von Willebrand Factor Antibody (HY-P86402). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • Experimental Validation Results for von Willebrand Factor Antibody (YA6094)
  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse spleen tissue (lane2, 20μg) and Rat spleen tissue (lane3, 20μg) using von Willebrand Factor Antibody (HY-P86402). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using von Willebrand Factor Antibody (YA6094). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86402, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • von Willebrand Factor Antibody (YA6094) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling von Willebrand Factor with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with von Willebrand Factor Antibody (HY-P86402) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • von Willebrand Factor Antibody (YA6094) Product Image WB
  • von Willebrand Factor Antibody (YA6094) Product Image IHC-P
  • von Willebrand Factor Antibody (YA6094) Product Image IHC-P
  • von Willebrand Factor Antibody (YA6094) Product Image IHC-P
  • von Willebrand Factor Antibody (YA6094) Product Image IHC-P
  • von Willebrand Factor Antibody (YA6094) Product Image IHC-P
  • von Willebrand Factor Antibody (YA6094) Product Image IHC-P
  • von Willebrand Factor Antibody (YA6094) Product Image ICC
  • von Willebrand Factor Antibody (YA6094) Product Image ICC