hnRNP M Antibody (YA3034)

Cat. No.: HY-P83289: Data Sheet COA
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User Guide for Antibodies
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hnRNP M Antibody (YA3034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP M.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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hnRNP M Antibody (YA3034) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
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hnRNP M Antibody (YA3034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP M.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 78 kDa;

Observed band size: 78 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P52272

Gene ID

4670 [NCBI]

Immunogen

A synthetic peptide of human hnRNP M1-M4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and 293T (lane 4, 15 μg) using hnRNP M Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines

Subcellular Localization：Nucleus, nucleolus

Isoforms & Post-Translational Modification：P52272 has 3 isomers: P52272-1: 77516 Da (predicted); P52272-2: 73621 Da (predicted); P52272-3: Da (predicted).
Sumoylated

Subunit：Identified in the spliceosome C complex (PubMed:11991638). Interacts with PPIA/CYPA (PubMed:25678563)

Database

Entrez Gene: 4670 Human ; 76936 Mouse ; 116655 Rat

SwissProt: P52272 Human ; Q9D0E1 Mouse ; Q62826 Rat

OMIM: 160994 Human

Research Field

Tags & Cell Markers

Synonyms

CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

hnRNP M Antibody (YA3034) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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hnRNP M Antibody (YA3034)

hnRNP M Antibody (YA3034) Featured Recommendations:

Product Detail

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Background

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hnRNP M Antibody (YA3034) Related Classifications

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SAFETY DATA SHEET (SDS)

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