DGKZ Antibody (YA9787)
(Synonyms: DAGK5, DAGK6, DGK-ZETA, hDGKzeta, DGKZ)
Based on 1 Customer Validation
DGKZ Antibody (YA9787) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to DGKZ.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:500-2000 | 1:20-50 | 1:200-500 |
Product Details
DGKZ Antibody (YA9787) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to DGKZ.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 124,114 kDa;
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 104 kDa
Recombinant protein of human DGKZ/DGK-zeta
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis was performed on protein extracts (25 μg) from Rat brain (lane 2) and Jurkat (lane 3) using DGKZ antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
Background
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Function
DGKZ is a Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group. The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization. Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity)[1][2][3][4][5][6][7][8].
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Subcellular Localization
Nucleus; Cytoplasm, cytosol; Cell membrane; Cell projection, lamellipodium
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Expression
Tissue_Specificity: Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. -
Isoforms & Post-Translational Modification
DGKZ has 7 isoforms, Q13574-2: amino acid length is 928, molecular weight is 103981 Da (predicted); Q13574-1: amino acid length is 1117, molecular weight is 124128 Da (predicted); Q13574-3: amino acid length is 945, molecular weight is 106030 Da (predicted); Q13574-4: amino acid length is 929, molecular weight is 104109 Da (predicted); Q13574-5: amino acid length is 934, molecular weight is 104581 Da (predicted); Q13574-6: amino acid length is 906, molecular weight is 101230 Da (predicted); Q13574-7: amino acid length is 933, molecular weight is 104296 Da (predicted).
Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta. -
Subunit
Interacts (via PDZ-binding motif) with the PDZ domain of the syntrophin SNTG1 and that of SNX27.
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SwissProt ID
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Synonyms
DAGK5, DAGK6, DGK-ZETA, hDGKzeta, DGKZ
Documentation
References
[1]. Epand RM, et al. The alpha isoform of diacylglycerol kinase exhibits arachidonoyl specificity with alkylacylglycerol. Biochemistry. 2004 Nov 23;43(46):14778-83. [Content Brief]
[2]. Epand RM, et al. Substrate chirality and specificity of diacylglycerol kinases and the multisubstrate lipid kinase. Biochemistry. 2007 Dec 11;46(49):14225-31. [Content Brief]
[3]. Lung M, et al. Diacylglycerol kinase epsilon is selective for both acyl chains of phosphatidic acid or diacylglycerol. J Biol Chem. 2009 Nov 6;284(45):31062-73. [Content Brief]
[4]. Shulga YV, et al. Substrate specificity of diacylglycerol kinase-epsilon and the phosphatidylinositol cycle. FEBS Lett. 2011 Dec 15;585(24):4025-8. [Content Brief]
[5]. Gellett AM, et al. Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases. Biochem Biophys Res Commun. 2012 Jun 15;422(4):758-63. [Content Brief]
[6]. Sato M, et al. Evaluations of the selectivities of the diacylglycerol kinase inhibitors R59022 and R59949 among diacylglycerol kinase isozymes using a new non-radioactive assay method. Pharmacology. 2013;92(1-2):99-107. [Content Brief]
[7]. Ding L, et al. Alternative splicing of the human diacylglycerol kinase zeta gene in muscle. Proc Natl Acad Sci U S A. 1997 May 27;94(11):5519-24. [Content Brief]
[8]. Luo B, et al. Diacylglycerol kinase zeta regulates phosphatidylinositol 4-phosphate 5-kinase Ialpha by a novel mechanism. Cell Signal. 2004 Aug;16(8):891-7. [Content Brief]
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