TAX1BP1 Antibody (YA2248)(PBS only)

Cat. No.: HY-P82503A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

TAX1BP1 Antibody (YA2248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAX1BP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TAX1BP1 Antibody (YA2248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAX1BP1.

Host

Rabbit

Molecular Weight

Predicted band size: 91 kDa;

Observed band size: 91 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q86VP1

Gene ID

8887 [NCBI]

Immunogen

A synthetic peptide of human TRAF6BP

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation (PubMed:29940186, PubMed:30459273, PubMed:30909570). Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates (PubMed:17703191). Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN-beta production (PubMed:21885437). Also recruits A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways (PubMed:17703191). Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling (PubMed:27736772). As a macroautophagy/autophagy receptor, facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis (PubMed:26451915). Upon NBR1 recruitment to the SQSTM1-ubiquitin condensates, acts as the major recruiter of RB1CC1 to these ubiquitin condensates to promote their autophagic degradation (PubMed:33226137, PubMed:34471133). Mediates the autophagic degradation of other substrates including TICAM1 (PubMed:28898289)

Subcellular Localization：Cytoplasm; Mitochondrion; Preautophagosomal structure; Cytoplasmic vesicle, autophagosome

Expression：
Tissue_specificity:Expressed in all tissues tested

Isoforms & Post-Translational Modification：Q86VP1 has 4 isomers: Q86VP1-1: 90877 Da (predicted); Q86VP1-2: 86257 Da (predicted); Q86VP1-3: 65112 Da (predicted); Q86VP1-4: 68277 Da (predicted).
Phosphorylated in the C-terminal region by CHUK/IKKA leading to NF-kappa-B signaling down-regulation

Subunit：Homooligomer. Interacts with TNFAIP3. Interacts with STARD13. Interacts with MYO6 (PubMed:31371777). Interacts with TOM1; the interaction is indirect and is mediated by MYO6, which acts as a bridge between TOM1 and TAX1BP1 (PubMed:31371777). Interacts with MAVS; this interaction induces MAVS polyubiquitination (PubMed:27736772). Interacts with TNIP1 (PubMed:21885437). Interacts with TRAF6; this interaction mediates deubiquitination of TRAF6 and inhibition of NF-kappa-B activation (PubMed:17703191). Interacts with RIPK1; this interaction negatively regulates RIPK1 ubiquitination (PubMed:17703191). Interacts with NBR1 (PubMed:33226137, PubMed:34471133). Interacts with TBK1 (PubMed:33226137). Interacts with RB1CC1 (PubMed:33226137, PubMed:34471133). Interacts with SQSTM1 (PubMed:34471133). Interacts with AZI2 (PubMed:30459273). Interacts with TICAM1 and TRIM32; these interactions target TICAM1 to TAX1BP1-mediated selective autophagic degradation (PubMed:28898289)

Research Field

Signal Transduction

Synonyms

CALCOCO3; D6Ertd404e; D6Ertd772e; PRO0105; T6BP; TAX1BP1; tax1bp1b; TXBP151

Documentation

User Guide for Antibodies (1077 KB)